Table 1. Effects of Protease Inhibitors... | American Society of Hematology

Table 1.

Effects of Protease Inhibitors on Glucocorticoid- and Fludarabine-Induced Apoptosis

Patient No.	Control	zAPF^*	zVAD^†	MPS^‡	M + A	M + V	M + TPCK
1	0.6	ND	ND	14.8	ND	7.3	ND
2	21.8	ND	ND	56.4	ND	39.7	ND
3	5.7	ND	ND	10.2	ND	6.5	ND
4	9	ND	ND	12.4	ND	11.6	5.3
5	27.2	ND	ND	56.5	ND	64.4	22.4
6	26.4	ND	ND	52.6	ND	13.7	11.3
7	10.1	ND	ND	31.7	ND	17.9	40.8
8	0.9	ND	ND	83.4	2.4	83.2	5.5
9	4.4	ND	ND	59.8	0.8	53.3	13.4
10	7.6	ND	ND	16.3	8.6	17.1	ND
11	1.6	ND	ND	77.9	2.4	46.3	ND
12	10.5	0.6	10.4	52.6	0.6	30.2	ND
13	14.6	5.9	5.8	15.8	3.4	9	ND
14	58.8	0.9	12.9	66.4	1.9	46.7	ND
15	26.1	0.4	21.1	51.5	1.2	31.3	ND
16	21.7	1.5	29.6	49.8	1	41.7	ND
17	17.2	2.9	11.9	48	4	19.5	ND
18	22.3	0.3	26.6	76	0.2	33.9	ND
19	1.8	3.5	1.3	6.7	1.3	7.2	ND
20	32.7	ND	ND	57.2	2.2	ND	43
21	18.3	ND	ND	65.1	ND	11.6	ND
22	26.1	0.4	21.1	51.5	1.2	31.3	ND
23	21.7	1.5	29.6	49.8	1.0	41.7	ND
24	3.0	0.1	ND	15	0.2	ND	ND
25	3.0	0.1	ND	29.1	0.1	ND	ND

Patient No.	Control	zAPF^*	zVAD^†	MPS^‡	M + A	M + V	M + TPCK
1	0.6	ND	ND	14.8	ND	7.3	ND
2	21.8	ND	ND	56.4	ND	39.7	ND
3	5.7	ND	ND	10.2	ND	6.5	ND
4	9	ND	ND	12.4	ND	11.6	5.3
5	27.2	ND	ND	56.5	ND	64.4	22.4
6	26.4	ND	ND	52.6	ND	13.7	11.3
7	10.1	ND	ND	31.7	ND	17.9	40.8
8	0.9	ND	ND	83.4	2.4	83.2	5.5
9	4.4	ND	ND	59.8	0.8	53.3	13.4
10	7.6	ND	ND	16.3	8.6	17.1	ND
11	1.6	ND	ND	77.9	2.4	46.3	ND
12	10.5	0.6	10.4	52.6	0.6	30.2	ND
13	14.6	5.9	5.8	15.8	3.4	9	ND
14	58.8	0.9	12.9	66.4	1.9	46.7	ND
15	26.1	0.4	21.1	51.5	1.2	31.3	ND
16	21.7	1.5	29.6	49.8	1	41.7	ND
17	17.2	2.9	11.9	48	4	19.5	ND
18	22.3	0.3	26.6	76	0.2	33.9	ND
19	1.8	3.5	1.3	6.7	1.3	7.2	ND
20	32.7	ND	ND	57.2	2.2	ND	43
21	18.3	ND	ND	65.1	ND	11.6	ND
22	26.1	0.4	21.1	51.5	1.2	31.3	ND
23	21.7	1.5	29.6	49.8	1.0	41.7	ND
24	3.0	0.1	ND	15	0.2	ND	ND
25	3.0	0.1	ND	29.1	0.1	ND	ND

Apoptosis was quantified by PI staining and FACS analysis after 48 hours of culture.

Abbreviations: ND, not determined; MPS, methylprednisolone succinate; M + A, MPS + zAPFcmk; M + V, MPS + zVADfmk.

10 μmol/L zAPFcmk.

25 μmol/L zVADfmk.

10 μmol/L methylprednisolone.