Effect of Growth Factor Treatment on BM Progenitors
| Treatment Group . | CFU-GM (×103) . | CFU-GEMM (×102) . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | Day 1 . | Day 3 . | Day 5 . | Day 7 . | Day 1 . | Day 3 . | Day 5 . | Day 7 . |
| MSA | 87 ± 3* | 86 ± 15 | 78 ± 11 | 76 ± 9 | BD† | 15 ± 5 | 16 ± 7 | 19 ± 6 |
| FL | 109 ± 9 | 269 ± 50‡ | 295 ± 34‡ | 287 ± 55‡ | 25 ± 25 | 64 ± 18‡ | 89 ± 24‡ | 27 ± 3 |
| G-CSF | 97 ± 10 | 94 ± 6 | 86 ± 12 | ND | 34 ± 3 | 35 ± 4 | 24 ± 12 | ND |
| GM-CSF | 82 ± 18 | 93 ± 9 | 85 ± 12 | 80 ± 6 | 27 ± 9 | 24 ± 3 | 31 ± 7 | 27 ± 15 |
| FL + G-CSF | 108 ± 14 | 304 ± 13‡ | 54 ± 3 | 27 ± 2‡ | 26 ± 13 | 62 ± 10‡ | 15 ± 1 | BD |
| FL + GM-CSF | 75 ± 6 | 283 ± 27‡ | 268 ± 40‡ | 171 ± 26‡ | 50 ± 11 | 77 ± 28 | 67 ± 7‡ | 16 ± 7 |
| Treatment Group . | CFU-GM (×103) . | CFU-GEMM (×102) . | ||||||
|---|---|---|---|---|---|---|---|---|
| . | Day 1 . | Day 3 . | Day 5 . | Day 7 . | Day 1 . | Day 3 . | Day 5 . | Day 7 . |
| MSA | 87 ± 3* | 86 ± 15 | 78 ± 11 | 76 ± 9 | BD† | 15 ± 5 | 16 ± 7 | 19 ± 6 |
| FL | 109 ± 9 | 269 ± 50‡ | 295 ± 34‡ | 287 ± 55‡ | 25 ± 25 | 64 ± 18‡ | 89 ± 24‡ | 27 ± 3 |
| G-CSF | 97 ± 10 | 94 ± 6 | 86 ± 12 | ND | 34 ± 3 | 35 ± 4 | 24 ± 12 | ND |
| GM-CSF | 82 ± 18 | 93 ± 9 | 85 ± 12 | 80 ± 6 | 27 ± 9 | 24 ± 3 | 31 ± 7 | 27 ± 15 |
| FL + G-CSF | 108 ± 14 | 304 ± 13‡ | 54 ± 3 | 27 ± 2‡ | 26 ± 13 | 62 ± 10‡ | 15 ± 1 | BD |
| FL + GM-CSF | 75 ± 6 | 283 ± 27‡ | 268 ± 40‡ | 171 ± 26‡ | 50 ± 11 | 77 ± 28 | 67 ± 7‡ | 16 ± 7 |
Mice were treated daily (SC) with 1 μg MSA (control) or MSA plus each growth factor (10 μg/d) for up to 7 days. On days 1 (24 hours after the first injection), 3, 5, and 7, mice (N = 3) were killed and BM (2 femurs/mouse) was assessed for CFU numbers. BM samples from each animal were plated separately at various cell densities depending on previous growth factor treatment. Only colonies consisting of 50 or more cells were scored. Day 1 CFU-GEMM results from MSA-treated mice were below detectable levels, thus they could not be compared against growth factor-treated mice for statistical analysis.
Abbreviation: ND, not done.
Mean ± SEM per 2 femurs.
BD, below limit of detection (<500 CFU-GEMM/2 femurs).
Significantly increased or decreased when compared to MSA control (P < .05).