Colony Growth After Retroviral FAA Transduction of CD34 Cells
| Condition (virus/MMC) . | BFU-E . | Colony No.3-150 . | CFU-GEMM . |
|---|---|---|---|
| . | . | CFU-GM . | . |
| A. Patient 1 | |||
| −Virus/−MMC | 1 | 15 | 1 |
| +Virus/−MMC | 0 | 30 | 3 |
| −Virus/+MMC | 0 | 8 | 0 |
| +Virus/+MMC | 0 | 25 | 2 |
| B. Patient 2 | |||
| −Virus/−MMC | 0 | 0 | 0 |
| +Virus/−MMC | 4 | 24 | 16 |
| C. Patient 3 | |||
| −Virus/−MMC | 10 | 40 | 7 |
| −Virus/+MMC | 0 | 6 | 0 |
| +FAA virus/−MMC | 26 | 115 | 12 |
| +FAA virus/+MMC | 14 | 50 | 8 |
| +FAC virus/−MMC | 26 | 46 | 6 |
| +FAC virus/+MMC | 1 | 10 | 0 |
| D. Umbilical cord blood | |||
| −Virus/−MMC | 20 | 52 | 16 |
| −Virus/+MMC | 22 | 48 | 14 |
| Condition (virus/MMC) . | BFU-E . | Colony No.3-150 . | CFU-GEMM . |
|---|---|---|---|
| . | . | CFU-GM . | . |
| A. Patient 1 | |||
| −Virus/−MMC | 1 | 15 | 1 |
| +Virus/−MMC | 0 | 30 | 3 |
| −Virus/+MMC | 0 | 8 | 0 |
| +Virus/+MMC | 0 | 25 | 2 |
| B. Patient 2 | |||
| −Virus/−MMC | 0 | 0 | 0 |
| +Virus/−MMC | 4 | 24 | 16 |
| C. Patient 3 | |||
| −Virus/−MMC | 10 | 40 | 7 |
| −Virus/+MMC | 0 | 6 | 0 |
| +FAA virus/−MMC | 26 | 115 | 12 |
| +FAA virus/+MMC | 14 | 50 | 8 |
| +FAC virus/−MMC | 26 | 46 | 6 |
| +FAC virus/+MMC | 1 | 10 | 0 |
| D. Umbilical cord blood | |||
| −Virus/−MMC | 20 | 52 | 16 |
| −Virus/+MMC | 22 | 48 | 14 |
The number of progenitor colonies (>50 cells/colony) measured at day 15 in methylcellulose culture. Results are expressed as colony no./2 × 104 CD34+ cells plated. Samples were performed in duplicate and results are expressed as mean colony number. The MMC concentration was 1.0 nmol/L in all experiments.