Engraftment of UCB Cells in Preconditioned SCID Mice
| Graft . | Graft Size* . | Chimeric-151 Mice/ Injected . | % Chimerism (CD45+ cells) . | Characteristics of Chimerism . | |||
|---|---|---|---|---|---|---|---|
| CD34+ Cells . | CD34+CD38− Cells . | ||||||
| Cells/ Mouse BM (×106) . | Expansion Factor . | Cells/ Mouse BM (×104) . | Expansion Factor . | ||||
| Unfractionated | 107 | 22/22 | 34.5 ± 19.3-152 | 1.1 ± 1.4-153 | 12.9 ± 19.2 | 1.84 ± 2.7 | 2.19 ± 4.92 |
| CD34− | 107 | 5/18 | 7.8 ± 8.6 | 0.1 ± 0.1 | 4.0 ± 3.7 | 0 | 0 |
| CD34+ | 105 | 35/38 | 20.4 ± 16.3 | 1.1 ± 0.9 | 14.1 ± 18.6 | 6.9 ± 13.2 | 10.8 ± 16.6¶ |
| CD34+CD38+ | 5 × 104 | 1/4 | 1.7 | 0 | 0 | 0 | 0 |
| CD34+CD38− | 5 × 103 | 4/6 | 18.4 ± 8.7 | 0.1 ± 0.1 | 18.6 ± 5.2 | 0 | 0¶ |
| Graft . | Graft Size* . | Chimeric-151 Mice/ Injected . | % Chimerism (CD45+ cells) . | Characteristics of Chimerism . | |||
|---|---|---|---|---|---|---|---|
| CD34+ Cells . | CD34+CD38− Cells . | ||||||
| Cells/ Mouse BM (×106) . | Expansion Factor . | Cells/ Mouse BM (×104) . | Expansion Factor . | ||||
| Unfractionated | 107 | 22/22 | 34.5 ± 19.3-152 | 1.1 ± 1.4-153 | 12.9 ± 19.2 | 1.84 ± 2.7 | 2.19 ± 4.92 |
| CD34− | 107 | 5/18 | 7.8 ± 8.6 | 0.1 ± 0.1 | 4.0 ± 3.7 | 0 | 0 |
| CD34+ | 105 | 35/38 | 20.4 ± 16.3 | 1.1 ± 0.9 | 14.1 ± 18.6 | 6.9 ± 13.2 | 10.8 ± 16.6¶ |
| CD34+CD38+ | 5 × 104 | 1/4 | 1.7 | 0 | 0 | 0 | 0 |
| CD34+CD38− | 5 × 103 | 4/6 | 18.4 ± 8.7 | 0.1 ± 0.1 | 18.6 ± 5.2 | 0 | 0¶ |
*Percentage CD34+ cells in the graft: unfractionated UCB, 0.7% to 3.1%; CD34+, 77% to 83%; and CD34−, 0.1% to 0.6%.
Mice are considered chimeric at greater than 1% CD45+cells.
Mean ± SD of chimeric mice, results of 5 UCB.
CD34+ BM cells, calculated on the assumption that 1 femur represents 8.5% of all BM cells.30
¶Significantly different from unfractionated graft (P = .03, Fisher's exact test).