Frequency Analysis of Repopulating Cells
| Graft* . | CD34+ Cell No. Transplanted† . | Mice Positive for CD34+CD38− (n) . | Mice Negative for CD34+CD38− (n) . | % Negative . |
|---|---|---|---|---|
| CD34+ | 1,660 | 0 | 2 | 100 |
| 8,300 | 0 | 2 | 100 | |
| 16,600 | 1 | 1 | 50 | |
| 80,000 | 10 | 12 | 55 | |
| 104,097 | 8 | 2 | 20 | |
| 166,000 | 3 | 0 | 0 | |
| Total | 3,200 | 0 | 2 | 100 |
| 9,600 | 0 | 3 | 100 | |
| 32,000 | 0 | 4 | 100 | |
| 60,000 | 2 | 3 | 60 | |
| 70,000 | 3 | 3 | 50 | |
| 111,000 | 2 | 1 | 33 | |
| 198,900 | 3 | 2 | 40 |
| Graft* . | CD34+ Cell No. Transplanted† . | Mice Positive for CD34+CD38− (n) . | Mice Negative for CD34+CD38− (n) . | % Negative . |
|---|---|---|---|---|
| CD34+ | 1,660 | 0 | 2 | 100 |
| 8,300 | 0 | 2 | 100 | |
| 16,600 | 1 | 1 | 50 | |
| 80,000 | 10 | 12 | 55 | |
| 104,097 | 8 | 2 | 20 | |
| 166,000 | 3 | 0 | 0 | |
| Total | 3,200 | 0 | 2 | 100 |
| 9,600 | 0 | 3 | 100 | |
| 32,000 | 0 | 4 | 100 | |
| 60,000 | 2 | 3 | 60 | |
| 70,000 | 3 | 3 | 50 | |
| 111,000 | 2 | 1 | 33 | |
| 198,900 | 3 | 2 | 40 |
*Only chimeric SCID mice (>1% CD45+ cells by flow cytometry) were included in the analysis.
n = 5 UCB, 69 mice transplanted.