Table 1.

Analysis of CFU-S From Mice Transplanted With Retrovirally Transduced Hematopoietic Cells

Exp. No.TiterCell SourceCationNo. of MiceNo. of CFU-Sβ PCR+ Colonies%
1 105 BM PB 2 12 10* 83 
2 104 BM PB 1 6 1 17 
3 105 FL PB 2 11 8 73 
4 105 BM PT 2 11 11 100 
Exp. No.TiterCell SourceCationNo. of MiceNo. of CFU-Sβ PCR+ Colonies%
1 105 BM PB 2 12 10* 83 
2 104 BM PB 1 6 1 17 
3 105 FL PB 2 11 8 73 
4 105 BM PT 2 11 11 100 

Hematopoietic cells were obtained, preincubated with growth factors, and then cocultured with β-globin retroviral producer cells. Lethally irradiated recipient mice were injected with transduced cells and 1-2 mice were killed 12 days posttransplantation. CFU-S were counted and DNA extracted. PCR specific for the transferred β-globin gene was performed on 0.5 μg of DNA from individual colonies.

Abbreviations: Exp, experiment; PB, polybrene; PT, protamine.

*

The 10 PCR-positive CFU-S are shown in Fig 2. Two PCR-negative CFU-S were omitted from the gel shown in Fig 2.

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