Human β -Globin Gene Transfer and Expression in Peripheral Blood of Primary and Secondary Recipient Mice
| Animal . | Recipient . | β-Globin . | Primer Extension . | Human β/Mouse βmaj (%) . |
|---|---|---|---|---|
| . | . | PCR . | . | . |
| Mouse 1 | Primary | + | − | − |
| 1A | Secondary | − | − | − |
| 1B | Secondary | − | − | − |
| Mouse 2 | Primary | − | − | − |
| 2A | Secondary | − | − | − |
| 2B | Secondary | − | − | − |
| Mouse 3 | Primary | + | + | 5 |
| 3A | Secondary | + | + | 8 |
| 3B | Secondary | + | + | 11 |
| Mouse 4 | Primary | + | + | 20 |
| 4A | Secondary | + | + | 3 |
| 4B | Secondary | + | + | 12 |
| Animal . | Recipient . | β-Globin . | Primer Extension . | Human β/Mouse βmaj (%) . |
|---|---|---|---|---|
| . | . | PCR . | . | . |
| Mouse 1 | Primary | + | − | − |
| 1A | Secondary | − | − | − |
| 1B | Secondary | − | − | − |
| Mouse 2 | Primary | − | − | − |
| 2A | Secondary | − | − | − |
| 2B | Secondary | − | − | − |
| Mouse 3 | Primary | + | + | 5 |
| 3A | Secondary | + | + | 8 |
| 3B | Secondary | + | + | 11 |
| Mouse 4 | Primary | + | + | 20 |
| 4A | Secondary | + | + | 3 |
| 4B | Secondary | + | + | 12 |
Four primary recipient mice from experiment 1 were killed 9 months posttransplantation and their marrow used to reconstitute secondary recipients. Peripheral blood samples of secondary recipients were analyzed for human β-globin gene transfer by PCR and gene expression by primer extension as described in Materials and Methods. Data are shown for each of the primary and secondary recipients. (+) indicates a signal for the human β-globin gene by PCR or mRNA by primer extension; (−) indicates no signal.