Migration of Hematopoietic Progenitor Cells on Surface Coated With Various Extracellular Matrix Proteins
| Coating . | No. of Cells per Field* . | |
|---|---|---|
| . | FDCP-Mix . | lin− Sca+ BM . |
| BSA | 2 ± 1 | 7 ± 4 |
| Hyaluronic acid | 4 ± 2 | 6 ± 2 |
| Fn | 55 ± 10† | 24 ± 9† |
| Lam | 23 ± 4† | 18 ± 4† |
| Collagen IV | 4 ± 1 | 8 ± 3 |
| Coating . | No. of Cells per Field* . | |
|---|---|---|
| . | FDCP-Mix . | lin− Sca+ BM . |
| BSA | 2 ± 1 | 7 ± 4 |
| Hyaluronic acid | 4 ± 2 | 6 ± 2 |
| Fn | 55 ± 10† | 24 ± 9† |
| Lam | 23 ± 4† | 18 ± 4† |
| Collagen IV | 4 ± 1 | 8 ± 3 |
FDCP-mix A4 cells (5,000 per well) or lin− Sca+ BM cells (2,000 per well) were seeded in IMDM/FCS and 100 U/mL IL-3 into migration assays on 15°C tilted plates as described in Materials and Methods. Results are represented as means ± SD of three independent experiments.
Indicates statistically significant difference from control (Student's t-test, P < .05).