In Myeloma, Circulating and BM-Localized Plasma Cells Lack a Functional Transporter for Rh123
| Tissue (no. donors) . | % CsA-Sensitive Dye Efflux . | |||||
|---|---|---|---|---|---|---|
| . | CD38hi . | . | CD4+ . | . | CD8+ . | |
| MM BM | ||||||
| Unt (9) | 8 ± 2‡ | 26 ± 14 | 53 ± 6 | |||
| Tr (14) | 11 ± 3‡ | 44 ± 5 | 53 ± 4 | |||
| Terminal plasma cell leukemia | ||||||
| PBMC (4) | 17 ± 4† | 31 ± 1 | 45 ± 5 | |||
| MGUS BM (3) | 54 ± 5ρ | 21 ± 10 | 51 ± 5 | |||
| CD11B+ | CD4+ | CD8+ | Monocytes | |||
| MM PB | ||||||
| Unt (10) | 62 ± 5 | 30 ± 3 | 54 ± 3 | 6 ± 4 | ||
| Tr (27) | 43 ± 4* | 27 ± 2 | 51 ± 3 | 4 ± 2 | ||
| Off Tr (30) | 57 ± 3 | 30 ± 2 | 54 ± 3 | 6 ± 2 | ||
| MGUS PBMC (7) | 53 ± 5 | 34 ± 3 | 69 ± 4 | 13 ± 6 | ||
| Tissue (no. donors) . | % CsA-Sensitive Dye Efflux . | |||||
|---|---|---|---|---|---|---|
| . | CD38hi . | . | CD4+ . | . | CD8+ . | |
| MM BM | ||||||
| Unt (9) | 8 ± 2‡ | 26 ± 14 | 53 ± 6 | |||
| Tr (14) | 11 ± 3‡ | 44 ± 5 | 53 ± 4 | |||
| Terminal plasma cell leukemia | ||||||
| PBMC (4) | 17 ± 4† | 31 ± 1 | 45 ± 5 | |||
| MGUS BM (3) | 54 ± 5ρ | 21 ± 10 | 51 ± 5 | |||
| CD11B+ | CD4+ | CD8+ | Monocytes | |||
| MM PB | ||||||
| Unt (10) | 62 ± 5 | 30 ± 3 | 54 ± 3 | 6 ± 4 | ||
| Tr (27) | 43 ± 4* | 27 ± 2 | 51 ± 3 | 4 ± 2 | ||
| Off Tr (30) | 57 ± 3 | 30 ± 2 | 54 ± 3 | 6 ± 2 | ||
| MGUS PBMC (7) | 53 ± 5 | 34 ± 3 | 69 ± 4 | 13 ± 6 | ||
BMC or PBMC were loaded with Rh123, allowed to efflux dye for 3 hours followed by staining for surface phenotype as indicated using PE conjugated MoAbs. Monocytes were detected using CD14-PE.
Abbreviation: NA, not applicable.
P = .02.
P < .001.
P < .0001 all as compared to untreated MM blood B cells.
ρ P = .0001 as compared to untreated MM BM plasma cells.