Transduction Efficiency and Peak Fluorescence Ratio of Different Cell Lines Transduced With PA317/EGFP1 Retroviral Vectors
| Cell Line . | Transduction Efficiency (%)* . | Peak Fluorescence Ratio† . |
|---|---|---|
| NIH/3T3 | 73 | 560 |
| HeLa | 9 | 48 |
| J744 A.1 | 65 | 64 |
| K562 | 16 | 489 |
| TF-1 | 7 | 374 |
| Cell Line . | Transduction Efficiency (%)* . | Peak Fluorescence Ratio† . |
|---|---|---|
| NIH/3T3 | 73 | 560 |
| HeLa | 9 | 48 |
| J744 A.1 | 65 | 64 |
| K562 | 16 | 489 |
| TF-1 | 7 | 374 |
All transductions were made by seeding 4 × 104 cells on 1.6-cm culture dishes for 24 hours and then incubating them with 100 μL of vector-containing supernatant and 4 μg/mL protamine sulphate for 48 hours more.
The ratio obtained by dividing the peak fluorescence channel of the transduced cells by the control cell peak channel.