R123 Dye Efflux Assay of P-gp Activity in PBMC
| Category . | Patient No. . | High Efflux Population %R123 Efflux . | Low Efflux Population %R123 Efflux . |
|---|---|---|---|
| Asymptomatic | 1 | 0 | 11 |
| 2 | 88 | 6 | |
| 3 | 90 | 3 | |
| 4 | 85 | 1 | |
| 5 | 83 | 1 | |
| 6 | 92 | 10 | |
| Average | 73 | 5 | |
| TSP/HAM | 7 | 92 | 7 |
| 8 | 86 | 15 | |
| Average | 89 | 11 | |
| ATLL | 9 | 90 | 11 |
| Lymphoma | 10 | 87 | 2 |
| Overall HTLV+ Average: | 79 | 7 | |
| Asymptomatic No. 6 + 5 μmol/L reserpine | 0 | 27 | |
| Asymptomatic No. 6 + 5 μmol/L verapamil | 0 | 25 | |
| Non–HTLV-infected | 1 | 0 | 22 |
| 2 | 0 | 12 | |
| 3 | 0 | 2 | |
| 4 | 0 | 3 | |
| 5 | 0 | 15 | |
| 6 | 0 | 17 | |
| Overall HTLV− Average | 0 | 12 |
| Category . | Patient No. . | High Efflux Population %R123 Efflux . | Low Efflux Population %R123 Efflux . |
|---|---|---|---|
| Asymptomatic | 1 | 0 | 11 |
| 2 | 88 | 6 | |
| 3 | 90 | 3 | |
| 4 | 85 | 1 | |
| 5 | 83 | 1 | |
| 6 | 92 | 10 | |
| Average | 73 | 5 | |
| TSP/HAM | 7 | 92 | 7 |
| 8 | 86 | 15 | |
| Average | 89 | 11 | |
| ATLL | 9 | 90 | 11 |
| Lymphoma | 10 | 87 | 2 |
| Overall HTLV+ Average: | 79 | 7 | |
| Asymptomatic No. 6 + 5 μmol/L reserpine | 0 | 27 | |
| Asymptomatic No. 6 + 5 μmol/L verapamil | 0 | 25 | |
| Non–HTLV-infected | 1 | 0 | 22 |
| 2 | 0 | 12 | |
| 3 | 0 | 2 | |
| 4 | 0 | 3 | |
| 5 | 0 | 15 | |
| 6 | 0 | 17 | |
| Overall HTLV− Average | 0 | 12 |
Summary of R123 efflux assay data. With one exception, all HTLV-infected subjects showed a high efflux population of cells, irrespective of clinical status, indicating elevated P-gp activity (Fig2). The one exception (asymptomatic subject no. 1) was confirmed HTLV-I seropositive and genome-positive by PCR; it is presently unclear what distinguishes this subject from all of the others examined. In contrast, all of the non–HTLV-infected subjects examined are P-gp negative and show only a single low efflux population of cells (Fig2).