RT-PCR Analysis of Retrovirus Receptor mRNA in Human HSC
| . | AmphoR mRNA . | GaLVR mRNA . |
|---|---|---|
| Fetal liver | ||
| Lin− CD34+ CD38+ (2) | 0.31 ± 0.04* | 0.38 ± 0.41 |
| Lin−CD34+ CD38− (2) | 0.22 ± 0.13 | 0.28 ± 0.28 |
| Cord blood | ||
| Lin−CD34+ CD38+ (Fresh) (3) | 0.38 ± 0.34 | 0.08 ± 0.04 |
| Lin− CD34+CD38− (Fresh) (3) | 0.13 ± 0.05 | 0.07 ± 0.03 |
| Lin− CD34+ CD38+ (Frozen) (3) | 1.54 ± 1.61 | Not detected |
| Lin− CD34+ CD38− (Frozen) (3) | 1.55 ± 0.61†‡ | Not detected |
| Bone marrow | ||
| Lin− CD34+CD38+ (5) | 0.20 ± 0.20 | 0.22 ± 0.22 |
| Lin− CD34+ CD38− (4) | 0.07 ± 0.10 | 0.15 ± 0.14 |
| FR20 Lin−CD34+ CD38− (5) | 0.10 ± 0.10 | 0.01 ± 0.01 |
| FR25 Lin− CD34+CD38− (5) | 0.03 ± 0.02 | 0.02 ± 0.02 |
| FR30 Lin− CD34+ CD38− (5) | 0.05 ± 0.06 | 0.03 ± 0.03 |
| FR35 Lin−CD34+ CD38− (5) | 0.07 ± 0.06 | 0.05 ± 0.04 |
| FR40 Lin− CD34+CD38− (5) | 0.12 ± 0.15 | 0.10 ± 0.20 |
| HeLa cells (5) | 1.0 | 1.0 |
| . | AmphoR mRNA . | GaLVR mRNA . |
|---|---|---|
| Fetal liver | ||
| Lin− CD34+ CD38+ (2) | 0.31 ± 0.04* | 0.38 ± 0.41 |
| Lin−CD34+ CD38− (2) | 0.22 ± 0.13 | 0.28 ± 0.28 |
| Cord blood | ||
| Lin−CD34+ CD38+ (Fresh) (3) | 0.38 ± 0.34 | 0.08 ± 0.04 |
| Lin− CD34+CD38− (Fresh) (3) | 0.13 ± 0.05 | 0.07 ± 0.03 |
| Lin− CD34+ CD38+ (Frozen) (3) | 1.54 ± 1.61 | Not detected |
| Lin− CD34+ CD38− (Frozen) (3) | 1.55 ± 0.61†‡ | Not detected |
| Bone marrow | ||
| Lin− CD34+CD38+ (5) | 0.20 ± 0.20 | 0.22 ± 0.22 |
| Lin− CD34+ CD38− (4) | 0.07 ± 0.10 | 0.15 ± 0.14 |
| FR20 Lin−CD34+ CD38− (5) | 0.10 ± 0.10 | 0.01 ± 0.01 |
| FR25 Lin− CD34+CD38− (5) | 0.03 ± 0.02 | 0.02 ± 0.02 |
| FR30 Lin− CD34+ CD38− (5) | 0.05 ± 0.06 | 0.03 ± 0.03 |
| FR35 Lin−CD34+ CD38− (5) | 0.07 ± 0.06 | 0.05 ± 0.04 |
| FR40 Lin− CD34+CD38− (5) | 0.12 ± 0.15 | 0.10 ± 0.20 |
| HeLa cells (5) | 1.0 | 1.0 |
The number of samples are shown in parentheses. Receptor mRNA levels were normalized to the level of β2-m mRNA in the same sample. For quantification, the receptor mRNA levels were compared with the level of the receptor mRNA in unfractionated BM.
Mean ± SD of the mRNA levels relative to the level in HeLa cells.
P < .005 compared with Lin− CD34+CD38− BM cells.
P < .005 compared with fresh Lin−CD34+ CD38− cord blood cells.