RT-PCR Analysis of Retrovirus Receptor mRNA in BM HSC From Untreated and G-CSF– and SCF-Treated Mice
| . | amphoR . | ecoR . |
|---|---|---|
| Untreated mice | ||
| Unfractionated (9) | 0.49 ± 0.12* | 1.16 ± 0.47 |
| FR25 Lin− c-kitHI (3) | 0.05 ± 0.04† | 0.49 ± 0.25 |
| FR30 Lin− c-kitHI(2) | 0.12 ± 0.04† | Not done |
| FR35 Lin−c-kitHI (7) | 0.14 ± 0.02† | 1.10 ± 0.33 |
| Cytokine-injected mice‡ | ||
| Unfractionated (3) | 0.86 ± 0.23† | 1.53 ± 0.51 |
| FR25 Lin−c-kitHI (2) | 0.39 ± 0.37 | 0.61 ± 0.33 |
| FR30 Lin− c-kitHI (2) | 0.30 ± 0.20 | 0.67 ± 0.35 |
| FR35 Lin−c-kitHI (2) | 0.39 ± 0.06 | 0.81 ± 0.05 |
| NIH-3T3 | 1.0 | 1.0 |
| . | amphoR . | ecoR . |
|---|---|---|
| Untreated mice | ||
| Unfractionated (9) | 0.49 ± 0.12* | 1.16 ± 0.47 |
| FR25 Lin− c-kitHI (3) | 0.05 ± 0.04† | 0.49 ± 0.25 |
| FR30 Lin− c-kitHI(2) | 0.12 ± 0.04† | Not done |
| FR35 Lin−c-kitHI (7) | 0.14 ± 0.02† | 1.10 ± 0.33 |
| Cytokine-injected mice‡ | ||
| Unfractionated (3) | 0.86 ± 0.23† | 1.53 ± 0.51 |
| FR25 Lin−c-kitHI (2) | 0.39 ± 0.37 | 0.61 ± 0.33 |
| FR30 Lin− c-kitHI (2) | 0.30 ± 0.20 | 0.67 ± 0.35 |
| FR35 Lin−c-kitHI (2) | 0.39 ± 0.06 | 0.81 ± 0.05 |
| NIH-3T3 | 1.0 | 1.0 |
The number of samples are shown in parentheses. Receptor mRNA levels were quantified based on the level of β2-m mRNA in the same sample. They were then normalized to the levels in NIH-3T3 receptor mRNA and compared with the level of the receptor mRNA in unfractionated BM.
Mean ± SD of the mRNA levels relative to the level in NIH-3T3 cells.
P < .01 compared with unfractionated control BM.
Cytokine-injected mice received 200 μg G-CSF/kg/d and 50 μg SCF/kg/d for 5 days. BM cells were collected 14 days later.