Cytokine Mixtures Used to Stimulate the Proliferation and Differentiation of Candidate HSC
| . | . | . | Percentages of Viable Cells With the Following Phenotype . | |||
|---|---|---|---|---|---|---|
| Cytokine Conditions . | Number of Expts . | Fold Increase* . | 34− . | 34+45lo71lo . | 34+45lo71hi . | 34+45hi71hi . |
| SCF | 3 | 0.27 ± 0.14-151 | 7 ± 4 | 79 ± 6 | 4 ± 2 | 1 ± 1 |
| FL | 2 | 0.21, 0.15-151 | 20, 11 | 62, 66 | 3, 13 | 1, 4 |
| IL-3 | 3 | 0.56 ± 0.17 | 46 ± 14 | 29 ± 15 | 19 ± 6 | 3 ± 2 |
| SCF + FL | 3 | 0.43 ± 0.17 | 9 ± 4 | 56 ± 14 | 14 ± 7 | 12 ± 6 |
| SCF + IL-3 | 4 | 1.48 ± 0.88 | 31 ± 17 | 47 ± 19 | 24 ± 9 | 8 ± 4 |
| FL + IL-3 | 2 | 2.9, 1.9 | 17, 57 | 31, 13 | 33, 22 | 14, 6 |
| SCF + IL-3 + FL | 5 | 1.96 ± 1.05 | 24 ± 14 | 19 ± 9 | 29 ± 10 | 23 ± 9 |
| . | . | . | Percentages of Viable Cells With the Following Phenotype . | |||
|---|---|---|---|---|---|---|
| Cytokine Conditions . | Number of Expts . | Fold Increase* . | 34− . | 34+45lo71lo . | 34+45lo71hi . | 34+45hi71hi . |
| SCF | 3 | 0.27 ± 0.14-151 | 7 ± 4 | 79 ± 6 | 4 ± 2 | 1 ± 1 |
| FL | 2 | 0.21, 0.15-151 | 20, 11 | 62, 66 | 3, 13 | 1, 4 |
| IL-3 | 3 | 0.56 ± 0.17 | 46 ± 14 | 29 ± 15 | 19 ± 6 | 3 ± 2 |
| SCF + FL | 3 | 0.43 ± 0.17 | 9 ± 4 | 56 ± 14 | 14 ± 7 | 12 ± 6 |
| SCF + IL-3 | 4 | 1.48 ± 0.88 | 31 ± 17 | 47 ± 19 | 24 ± 9 | 8 ± 4 |
| FL + IL-3 | 2 | 2.9, 1.9 | 17, 57 | 31, 13 | 33, 22 | 14, 6 |
| SCF + IL-3 + FL | 5 | 1.96 ± 1.05 | 24 ± 14 | 19 ± 9 | 29 ± 10 | 23 ± 9 |
*Represents the fold increase in cell number after 5 days in culture; calculated by dividing the number of viable cells recovered at day 5 by the number of cells seeded on day 0; a value less than 1 indicates a decrease in cell number.
Values represent the mean ± SD for experiments performed three times or more; for experiments performed only twice, both values are reported.