Increase in Hb Oxidation Rate Due to GSH
Experimental Condition . | n . | Hb Oxidation Rate (μmol/L/min) . |
---|---|---|
Hb at 1 mg/mL (62 μmol/L heme) | ||
No GSH | 6 | .029 ± .002 |
+ 125 μmol/L GSH | 6 | .034 ± .005 |
+ 250 μmol/L GSH | 6 | .041 ± .011 |
+ 500 μmol/L GSH | 6 | .071 ± .022 |
Hb at 10 mg/mL (620 μmol/L heme) | ||
No GSH | 6 | .028 ± .001 |
+ 125 μmol/L GSH | 6 | .029 ± .005 |
+ 250 μmol/L GSH | 6 | .027 ± .004 |
+ 500 μmol/L GSH | 6 | .028 ± .002 |
+ 1250 μmol/L GSH | 3 | .033 ± .008 |
+ 2500 μmol/L GSH | 3 | .057 ± .002 |
+ 5000 μmol/L GSH | 3 | .085 ± .004 |
Experimental Condition . | n . | Hb Oxidation Rate (μmol/L/min) . |
---|---|---|
Hb at 1 mg/mL (62 μmol/L heme) | ||
No GSH | 6 | .029 ± .002 |
+ 125 μmol/L GSH | 6 | .034 ± .005 |
+ 250 μmol/L GSH | 6 | .041 ± .011 |
+ 500 μmol/L GSH | 6 | .071 ± .022 |
Hb at 10 mg/mL (620 μmol/L heme) | ||
No GSH | 6 | .028 ± .001 |
+ 125 μmol/L GSH | 6 | .029 ± .005 |
+ 250 μmol/L GSH | 6 | .027 ± .004 |
+ 500 μmol/L GSH | 6 | .028 ± .002 |
+ 1250 μmol/L GSH | 3 | .033 ± .008 |
+ 2500 μmol/L GSH | 3 | .057 ± .002 |
+ 5000 μmol/L GSH | 3 | .085 ± .004 |
Results are shown as the mean ± SD. Conditions are as for Table1, with the inclusion of 10 μmol/L DTPA in each case and with variable [GSH] and [Hb] as shown.