Table 1. Oligonucleotide primer... | American Society of Hematology

Table 1.

Oligonucleotide primer sequences

Targets		Primer sequence	Annealing temperature	Cycle no.
β-actin	S	5′-TGCTATCCAGGCTGTGCTAT-3′	55°C	20
	AS	5′-GATGGAGTTGAAGGTAGTTT-3′
Fas	S	5′-TAGAAGCTTCGGAGGATTGCTCAACAAC-3′	60°C	28
	AS	5′-TAGGAATTCTTGGTATTCTGGGTCCG-3′
Fas-L	S	5′-CAAGTCCAACTCAAGGTCCATGCC-3′	60°C	30
	AS	5′-CAGAGAGAGCTCAGATACGTTGAC-3′

Targets		Primer sequence	Annealing temperature	Cycle no.
β-actin	S	5′-TGCTATCCAGGCTGTGCTAT-3′	55°C	20
	AS	5′-GATGGAGTTGAAGGTAGTTT-3′
Fas	S	5′-TAGAAGCTTCGGAGGATTGCTCAACAAC-3′	60°C	28
	AS	5′-TAGGAATTCTTGGTATTCTGGGTCCG-3′
Fas-L	S	5′-CAAGTCCAACTCAAGGTCCATGCC-3′	60°C	30
	AS	5′-CAGAGAGAGCTCAGATACGTTGAC-3′

S indicates sense; AS, antisense orientation.