Equilibrium binding of 125I–GM-CSF to BaF-3 cells transfected with GMR constructs
| Clone . | KD, pM . | BMax, pM . | Receptor/cell . |
|---|---|---|---|
| α/β-GMR | 1800 | 43.1 | 2611 |
| α/β-GMR + β-GMR | 200 | 10.9 | 504 |
| α/β-GMR + β-GMRIT | 218 | 12.5 | 603 |
| 2570 | 59.6 | 2872 |
| Clone . | KD, pM . | BMax, pM . | Receptor/cell . |
|---|---|---|---|
| α/β-GMR | 1800 | 43.1 | 2611 |
| α/β-GMR + β-GMR | 200 | 10.9 | 504 |
| α/β-GMR + β-GMRIT | 218 | 12.5 | 603 |
| 2570 | 59.6 | 2872 |
Data represent mean values of 4 (α/β-GMR) and 2 (α/β-GMR + β-GMR) independent experiments; α/β-GMR + β-GMRIT cells (clone 3) were analyzed once.
KD indicates equilibrium dissociation constant; BMax, density of binding sites.