Induction of transforming growth factor-β and tumor necrosis factor-α in bone marrow stroma stimulated with substance-P(1-4)
| Stimulus* . | TGF-β (pg/mL) results . | TNF-α (pM) results . | ||
|---|---|---|---|---|
| 1st stimulation . | 2nd stimulation . | 1st stimulation . | 2nd stimulation . | |
| Unstimulated | < 5 | < 5 | 0.01 ± 10−4 | 0.015 ± 10−4 |
| SCF† | < 5 | 0.023 ± 10−4 | ||
| SP(1-4)‡ | 80 ± 81-155 | 31 ± 21-155 | ||
| SCF‡ | < 5 | 0.028 ± 10−4 | ||
| SP(1-4)† | 90 ± 151-155 | 40 ± 41-155 | ||
| SP(1-4)1-153 | 64 ± 41-155 | 36 ± 61-155 | ||
| SCF1-153 | < 5 | 0.025 ± 10−4 | ||
| Stimulus* . | TGF-β (pg/mL) results . | TNF-α (pM) results . | ||
|---|---|---|---|---|
| 1st stimulation . | 2nd stimulation . | 1st stimulation . | 2nd stimulation . | |
| Unstimulated | < 5 | < 5 | 0.01 ± 10−4 | 0.015 ± 10−4 |
| SCF† | < 5 | 0.023 ± 10−4 | ||
| SP(1-4)‡ | 80 ± 81-155 | 31 ± 21-155 | ||
| SCF‡ | < 5 | 0.028 ± 10−4 | ||
| SP(1-4)† | 90 ± 151-155 | 40 ± 41-155 | ||
| SP(1-4)1-153 | 64 ± 41-155 | 36 ± 61-155 | ||
| SCF1-153 | < 5 | 0.025 ± 10−4 | ||
BM stroma was stimulated with 10 nM SP(1-4) or 10 ng/mL SCF (first stimulation). After 16 hours, cells were harvested or cultures were restimulated for 16 hours (second stimulation) with the same amount or concentration of SP(1-4) or stem cell factor (SCF). Transforming growth factor (TGF)-β and tumor necrosis factor (TNF)-α levels were determined in the total volume of supernatants and cell extracts, obtained from one flask of stromal cells. The results are represented as the mean ± SD of the total concentration (secreted and cell-bound/106 stromal cells).
TGF indicates transforming growth factor; TNF, tumor necrosis factor; SCF, stem cell factor; SP, substance P.
The amounts used in the stimulations were 10 nM for SP(1-4) and 10 ng/mL for SCF.
First stimulation.
Second stimulation after a first stimulation with SCF.
Second stimulation after a first stimulation with SP(1-4).
P < .05 versus unstimulated or SCF.