Induction of 12/15-LOX Activity in Murine Red Blood Cells During Experimental Anemia
| Animals . | 12/15-LOX Activity (μg 12-HETE/mL red cells × 15 minutes) . | S/R Ratio of 12-HETE . |
|---|---|---|
| Control mice (no PH) | 0 | ND |
| Anemic mice (3.5 mg PH/kg) | 19.8 | 90:10 |
| Anemic mice (7 mg PH/kg) | 91.5 | >95:5 |
| Anemic mice (14 mg PH/kg) | 121.9 | >95:5 |
| Animals . | 12/15-LOX Activity (μg 12-HETE/mL red cells × 15 minutes) . | S/R Ratio of 12-HETE . |
|---|---|---|
| Control mice (no PH) | 0 | ND |
| Anemic mice (3.5 mg PH/kg) | 19.8 | 90:10 |
| Anemic mice (7 mg PH/kg) | 91.5 | >95:5 |
| Anemic mice (14 mg PH/kg) | 121.9 | >95:5 |
Female NMRI mice were injected a phenylhydracine (PH) solution subcutaneously at the dosage indicated in the table. The injection was repeated at 4 consecutive days, and development of reticulocytosis was followed by reticulocyte counting. The mice were allowed to recover for 3 days and were then sacrificed by diethylether inhalation. Blood was withdrawn from the V. cava inf., the cells were spun down and the buffy-coat containing the white cells, and platelets was removed. The red cells were washed with PBS, and 0.1 mL of packed cells were incubated with 0.1 mmol/L arachidonic acid for 15 minutes at 37°C. After lipid extraction the formation of 12- and 15-HETE was quantified by RP-HPLC.
Abbreviation: ND, not determined.