Addition of MCP-1 Has no Effect on the Cycling of Primitive CML Progenitors in LTC Adherent Layers
| Treatment . | % Kill After3H-Thymidine . | |
|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | |
| Regular feed | 58 ± 5 | 44 ± 9 |
| Mock feed | 49 ± 4 | 43 ± 5 |
| Regular feed + TGF-β | 8 ± 5 | 8 ± 9 |
| Regular feed + MCP-1 | 47 ± 12 | 30 ± 3 |
| Treatment . | % Kill After3H-Thymidine . | |
|---|---|---|
| Primitive BFU-E . | Primitive CFU-GM . | |
| Regular feed | 58 ± 5 | 44 ± 9 |
| Mock feed | 49 ± 4 | 43 ± 5 |
| Regular feed + TGF-β | 8 ± 5 | 8 ± 9 |
| Regular feed + MCP-1 | 47 ± 12 | 30 ± 3 |
TGF-β at 5 ng/mL and MCP-1 at 100 ng/mL. Results shown are the mean ±SEM of data from four independent experiments, each with cells from a different CML patient. The effect of TGF-β addition is significant (P < .01), whereas there is no significant effect of MCP-1 (P > .1).