Immunostaining Pattern of CD40L in Activated T-Cell Lines or Clones Established From Patients With XHIM
| Type . | No. of Genotypes . | polyAb . | MoAb 106 . | MoAb 1.7 . | MoAb 5c8 . | MoAb TRAP . | CD40-Ig . |
|---|---|---|---|---|---|---|---|
| 1 | 5 | (+) | (+) | (+) | (+) | (+) | (+) |
| 2 | 3 | (+) or + | (+) or + | (+) or + | (+) or + | (+) or + | − |
| 3 | 5 | (+) or + | (+) | (+) or − | (+) or − | (+) or − | − |
| 4 | 10 | (+) or + | − | − | − | − | − |
| 5 | 1 | − | − | − | − | − | − |
| Type . | No. of Genotypes . | polyAb . | MoAb 106 . | MoAb 1.7 . | MoAb 5c8 . | MoAb TRAP . | CD40-Ig . |
|---|---|---|---|---|---|---|---|
| 1 | 5 | (+) | (+) | (+) | (+) | (+) | (+) |
| 2 | 3 | (+) or + | (+) or + | (+) or + | (+) or + | (+) or + | − |
| 3 | 5 | (+) or + | (+) | (+) or − | (+) or − | (+) or − | − |
| 4 | 10 | (+) or + | − | − | − | − | − |
| 5 | 1 | − | − | − | − | − | − |
Activated T-cell lines or clones were exposed to one pAb, four MoAbs, and bCD40-Ig, and the immunostaining pattern (types 1-5) was determined (see Materials and Methods). The staining intensity of the patients' activated T-cell lines or clones was compared with that of normal T-cell lines or T-cell clones; +, equal to normal cells; (+), significantly reduced, but clearly positive; −, no binding.