Comparison of P falciparum G6PD mRNA Expression in Different Parasite Strains Grown in Normal and Deficient E
| Strain . | Maturation Stage . | N/D . |
|---|---|---|
| FCR-3 | Ring | 1.119 |
| Trophozoite | 0.869 | |
| Schizont | 1.050 | |
| KI | Ring | 0.955 |
| Trophozoite | 1.141 | |
| C10 | Ring | 1.163 |
| Trophozoite | 1.154 | |
| HB3B | Ring | 1.082 |
| Trophozoite | 0.972 | |
| T9/96 | Trophozoite | 0.976 |
| Strain . | Maturation Stage . | N/D . |
|---|---|---|
| FCR-3 | Ring | 1.119 |
| Trophozoite | 0.869 | |
| Schizont | 1.050 | |
| KI | Ring | 0.955 |
| Trophozoite | 1.141 | |
| C10 | Ring | 1.163 |
| Trophozoite | 1.154 | |
| HB3B | Ring | 1.082 |
| Trophozoite | 0.972 | |
| T9/96 | Trophozoite | 0.976 |
Northern blots were probed with K2O2, a 1.6-kb fragment ofP falciparum G6PD gene and with a 2.2-kb fragment ofP falciparum calmodulin gene. The hybridization signals obtained with both probes were quantified by densitometry. The K2O2 signals were normalized using calmodulin signals and the ratios: Normal (N)/Deficient (D) calculated. N, P falciparum (grown in normal E) normalized K2O2 signal; D, P falciparum (grown in deficient E) normalized K2O2 signal.