Effects of Suboptimal Concentration of Ionomycin on T-Cell Adhesive and Migratory Ability and Surface Expression of the Integrin Activation Reporter Epitope Recognized by the 24 MoAb
. | Medium . | Ionomycin (50 nmol/L) . |
---|---|---|
Adherent T cells | ||
Percentage | 24.2 ± 5 | 17.5 ± 44-150 |
Expression of 24 epitope (MFI) | 112.4 ± 25 | 44.9 ± 74-150 |
Migrated T cells | ||
Percentage | 4.4 ± 2 | 1.7 ± 14-150 |
Expression of 24 epitope (MFI) | 174.3 ± 34 | 86.2 ± 204-150 |
. | Medium . | Ionomycin (50 nmol/L) . |
---|---|---|
Adherent T cells | ||
Percentage | 24.2 ± 5 | 17.5 ± 44-150 |
Expression of 24 epitope (MFI) | 112.4 ± 25 | 44.9 ± 74-150 |
Migrated T cells | ||
Percentage | 4.4 ± 2 | 1.7 ± 14-150 |
Expression of 24 epitope (MFI) | 174.3 ± 34 | 86.2 ± 204-150 |
Data are expressed as the mean ± SEM of five separate experiments. The basal MFI of 24 epitope (29.8 ± 8) on resting T cells did not change after incubation with 50 nmol/L ionomycin for 30 minutes (31.1 ± 9, not significant).
P < .001 versus medium.