Table 1. Engraftment of CD34 Cells in... | American Society of Hematology

Table 1.

Engraftment of CD34⁺ Cells in Animals Preinjected With Irradiated CD34⁻ Cells and in Splenectomized Animals as Compared to Controls

Experiment^-150	Input	% CD45^-151 BM	% CD45^-151 Spleen	% CD45^-151 PB
I	CD34⁻	<1.0	<1.0	<1.0
	CD34⁺	18.2 ± 11.2	8.5 ± 4.7	3.7 ± 2.0
	CD34⁻ and CD34⁺	14.8 ± 4.1	13.5 ± 1.9	2.6 ± 0.2
II	CD34⁺	26.3 ± 1.2	nd	nd
	CD34⁺ Spl-X	19.4 ± 15.4	—	nd

Experiment^-150	Input	% CD45^-151 BM	% CD45^-151 Spleen	% CD45^-151 PB
I	CD34⁻	<1.0	<1.0	<1.0
	CD34⁺	18.2 ± 11.2	8.5 ± 4.7	3.7 ± 2.0
	CD34⁻ and CD34⁺	14.8 ± 4.1	13.5 ± 1.9	2.6 ± 0.2
II	CD34⁺	26.3 ± 1.2	nd	nd
	CD34⁺ Spl-X	19.4 ± 15.4	—	nd

Abbreviations: BM, bone marrow; PB, peripheral blood; nd, not done.

F0-150

Two independent experiments in which sublethally irradiated NOD/SCID mice were injected with CD34⁺ cells from a G-CSF–mobilized apheresis product. In experiment I, animals were either injected with 20 Gy irradiated monocyte-depleted (by plastic adherence for 30 minutes at 37°C) CD34⁻ cells (25 × 10⁶ per animal) alone, with CD34⁺ cells alone (9 × 10⁶ per animal), or with CD34⁻ cells followed by CD34⁺ cells four hours later (n = 2 per group). In experiment II, 12 × 10⁶ CD34⁺cells were injected in splenectomized or nonsplenectomized animals (n = 2 per group).

F0-151

Percentage human cells was determined by flow cytometry using an antibody specific for human CD45.

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