Distribution of Human Cells Among Hematopoietic Organs in Untreated and Mobilized NOD/SCID Mice
| Experiment . | CD34+ Cells Injected per Mouse (×106) . | n . | Treatment . | Human Cells per mL Blood (×105) . | Human Cells per Femur (×106) . | Human Cells per Spleen (×106) . |
|---|---|---|---|---|---|---|
| A | 13 | 4 | Control | 3.1 ± 0.8 | 7.7 ± 1.2 | nd |
| 5 | CY + G-CSF | 0.7 ± 0.2‡ | 0.7 ± 0.2‡ | nd | ||
| B | 15 | 3 | Control | 6.3 ± 0.9 | 8.7 ± 0.7 | 10.7 ± 2.3 |
| 3 | G-CSF | 18.5 ± 0.2† | 9.5 ± 0.3 | 9.7 ± 0.1 | ||
| C | 10 | 2 | Control | 1.7 ± 0.7 | 0.3 ± 0.2 | nd |
| 3 | G-CSF | 12.5 ± 2.7* | 0.8 ± 0.1 | nd | ||
| D | 25 | 2 | Control | 20.2 ± 4.9 | 5.7 ± 2.8 | 1.7 ± 1.2 |
| 2 | G-CSF | 29.1 ± 0.8 | 8.5 ± 1.0 | 5.9 ± 1.2 | ||
| 2 | G-CSF + SCF | 36.0 ± 6.9 | 9.0 ± 0.6 | 5.2 ± 1.6 | ||
| E | 16 | 3 | Control | 1.3 ± 1.0 | 3.4 ± 0.8 | 1.3 ± 0.3 |
| 4 | G-CSF + SCF | 1.1 ± 0.3 | 3.2 ± 0.9 | 2.6 ± 0.7 |
| Experiment . | CD34+ Cells Injected per Mouse (×106) . | n . | Treatment . | Human Cells per mL Blood (×105) . | Human Cells per Femur (×106) . | Human Cells per Spleen (×106) . |
|---|---|---|---|---|---|---|
| A | 13 | 4 | Control | 3.1 ± 0.8 | 7.7 ± 1.2 | nd |
| 5 | CY + G-CSF | 0.7 ± 0.2‡ | 0.7 ± 0.2‡ | nd | ||
| B | 15 | 3 | Control | 6.3 ± 0.9 | 8.7 ± 0.7 | 10.7 ± 2.3 |
| 3 | G-CSF | 18.5 ± 0.2† | 9.5 ± 0.3 | 9.7 ± 0.1 | ||
| C | 10 | 2 | Control | 1.7 ± 0.7 | 0.3 ± 0.2 | nd |
| 3 | G-CSF | 12.5 ± 2.7* | 0.8 ± 0.1 | nd | ||
| D | 25 | 2 | Control | 20.2 ± 4.9 | 5.7 ± 2.8 | 1.7 ± 1.2 |
| 2 | G-CSF | 29.1 ± 0.8 | 8.5 ± 1.0 | 5.9 ± 1.2 | ||
| 2 | G-CSF + SCF | 36.0 ± 6.9 | 9.0 ± 0.6 | 5.2 ± 1.6 | ||
| E | 16 | 3 | Control | 1.3 ± 1.0 | 3.4 ± 0.8 | 1.3 ± 0.3 |
| 4 | G-CSF + SCF | 1.1 ± 0.3 | 3.2 ± 0.9 | 2.6 ± 0.7 |
Distribution of the mean number (±SEM) of human cells in the bone marrow, spleen, and peripheral blood of mobilized and untreated NOD/SCID mice transplanted with mobilized human peripheral blood CD34+ cells. Experiments A through E are the same as those described in Fig 8. For treatment protocols, see legend of Fig 8. Numbers in control and treated animals have been statistically compared using the Student's t-test and the level of significance has been indicated.
P < .05.
P < .01.
P < .001.