Table 2.

Total RNA and protein yields from peripheral blood

Platelet yields, × 109
Blood, 10 mL
PRP*
Purification†
Yield
Sensitivity, copies/cell
Total RNA   3.1   2.1   1.0   1 μg   <0.1‡ 
Protein
 		 —
 		 —
 		 —
 		 2 mg
 		 12§
 
Platelet yields, × 109
Blood, 10 mL
PRP*
Purification†
Yield
Sensitivity, copies/cell
Total RNA   3.1   2.1   1.0   1 μg   <0.1‡ 
Protein
 		 —
 		 —
 		 —
 		 2 mg
 		 12§
 

— indicates not applicable.

*

Centrifugation step (platelet-rich plasma isolated by centrifugation).

†

Gel filtration with or without CD45+ leukocyte depletion.

‡

Calculated for microarray analysis after amplification step.

§

Calculated based on silver stain detection limits of 1 ng for a 50-kDa protein.

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