Table 1.

Influence of L-kynurenine treatment on KIR2DL1/S1+, KIR2DL3+, and KIR3DL1+ NK-cell subsets at different culture time points

KIRs analyzed*
KIR2DL1/S1
KIR2DL3
KIR3DL1
Total cell counts, cell/mL‡
Kynurenine treatment,*lasting from d 0 to d 2
 -
+
-
+
-
+
-
+
Day 0   37†  —   21   —   15   —   1.0 × 106  —  
Day 2   35   34   23   24   16   17   0.9 × 106  0.8 × 106 
Day 5
 		 33
 		 36
 		 20
 		 22
 		 18
 		 16
 		 1.2 × 106
 		 1 × 106
 
KIRs analyzed*
KIR2DL1/S1
KIR2DL3
KIR3DL1
Total cell counts, cell/mL‡
Kynurenine treatment,*lasting from d 0 to d 2
 -
+
-
+
-
+
-
+
Day 0   37†  —   21   —   15   —   1.0 × 106  —  
Day 2   35   34   23   24   16   17   0.9 × 106  0.8 × 106 
Day 5
 		 33
 		 36
 		 20
 		 22
 		 18
 		 16
 		 1.2 × 106
 		 1 × 106
 

—indicates not applicable.

*

Cells were cultured as indicated in Figure 4 and analyzed by immunofluorescence staining and cytofluorimetric analysis (data are referred to as 1 representative donor of 5 analyzed).

†

Numbers are referred to as the percentage of cells positive for the indicated KIRs within the whole NK-cell population analyzed.

‡

In these columns, numbers indicate the cell counts (cells/mL) at various time intervals and in the different culture conditions.

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