Table 2 Frequency and absolute numbers... | American Society of Hematology

Table 2

Frequency and absolute numbers of hematopoietic cells in Ptpn11^D61Y mice

	Bone marrow		Spleen
	Control	Ptpn11^D61Y	Control	Ptpn11^D61Y
Total cellularity, ×10⁶	54.7 ± 4.7	29.8 ± 6.9§	235 ± 10.6	1720 ± 368§
LSK, %*	0.06 ± 0.01	0.03 ± 0.01	0.010 ± 0.003	0.03 ± 0.01§
LSK, ×10³*	26.9 ± 6.3	7.1 ± 1.7§	20.9 ± 5.5	595 ± 207§
CMP, %†	0.07 ± 0.06	0.01 ± 0.002§	0.02 ± 0.004	0.05 ± 0.04
CMP, ×10³†	30.2 ± 4.7	3.15 ± 0.72§	32.7 ± 8.03	554 ± 418
GMP, %†	0.07 ± 0.01	0.030 ± 0.003§	0.009 ± 0.003	0.05 ± 0.01§
GMP, ×10³†	29.4 ± 5.4	7.80 ± 2.02§	18.9 ± 5.8	608 ± 167§
MEP, %†	0.08 ± 0.02	0.2 ± 0.06	0.06 ± 0.01	0.3 ± 0.1
MEP, ×10³†	31.5 ± 4.8	42.1 ± 1.7	136 ± 23.6	4340 ± 2200‖
CLP, %‡	0.04 ± 0.02	0.02 ± 0.01	NC	NC
CLP, ×10³‡	20.0 ± 12.4	3.11 ± 1.68	NC	NC
Mac-1⁺Gr1⁺ myeloid, %*	48.1 ± 2.7	42.5 ± 9.6	2.78 ± 0.5	20.4 ± 3.2§
Mac-1⁺Gr1⁺ myeloid, ×10⁶*	23.7 ± 4	10.1 ± 3.1§	6.1 ± 0.7	351 ± 57.7§
Ter119⁺ erythroid prog, %†	22.6 ± 4.3	22.6 ± 6.1	4.3 ± 0.7	25.5 ± 4.1§
Ter119⁺ erythroid prog, ×10⁶†	11.6 ± 3.9	5.6 ± 1.8	9.4 ± 1.8	535 ± 171§
CD3⁺ T cells, %‡	3.7 ± 0.4	5.9 ± 1.1	34.6 ± 2.2	11.1 ± 1.6§
CD3⁺ T cells, ×10⁶‡	2.1 ± 0.4	1.3 ± 0.2	86.6 ± 9.6	246 ± 83.3
CD19⁺B220⁺ B cells, %‡	15.3 ± 5.3	9.4 ± 1.2	48.1 ± 7.2	27.2 ± 8.4
CD19⁺B220⁺ B cells, ×10⁶‡	9 ± 3.9	2.1 ± 0.2	117 ± 13.1	505 ± 107§

	Bone marrow		Spleen
	Control	Ptpn11^D61Y	Control	Ptpn11^D61Y
Total cellularity, ×10⁶	54.7 ± 4.7	29.8 ± 6.9§	235 ± 10.6	1720 ± 368§
LSK, %*	0.06 ± 0.01	0.03 ± 0.01	0.010 ± 0.003	0.03 ± 0.01§
LSK, ×10³*	26.9 ± 6.3	7.1 ± 1.7§	20.9 ± 5.5	595 ± 207§
CMP, %†	0.07 ± 0.06	0.01 ± 0.002§	0.02 ± 0.004	0.05 ± 0.04
CMP, ×10³†	30.2 ± 4.7	3.15 ± 0.72§	32.7 ± 8.03	554 ± 418
GMP, %†	0.07 ± 0.01	0.030 ± 0.003§	0.009 ± 0.003	0.05 ± 0.01§
GMP, ×10³†	29.4 ± 5.4	7.80 ± 2.02§	18.9 ± 5.8	608 ± 167§
MEP, %†	0.08 ± 0.02	0.2 ± 0.06	0.06 ± 0.01	0.3 ± 0.1
MEP, ×10³†	31.5 ± 4.8	42.1 ± 1.7	136 ± 23.6	4340 ± 2200‖
CLP, %‡	0.04 ± 0.02	0.02 ± 0.01	NC	NC
CLP, ×10³‡	20.0 ± 12.4	3.11 ± 1.68	NC	NC
Mac-1⁺Gr1⁺ myeloid, %*	48.1 ± 2.7	42.5 ± 9.6	2.78 ± 0.5	20.4 ± 3.2§
Mac-1⁺Gr1⁺ myeloid, ×10⁶*	23.7 ± 4	10.1 ± 3.1§	6.1 ± 0.7	351 ± 57.7§
Ter119⁺ erythroid prog, %†	22.6 ± 4.3	22.6 ± 6.1	4.3 ± 0.7	25.5 ± 4.1§
Ter119⁺ erythroid prog, ×10⁶†	11.6 ± 3.9	5.6 ± 1.8	9.4 ± 1.8	535 ± 171§
CD3⁺ T cells, %‡	3.7 ± 0.4	5.9 ± 1.1	34.6 ± 2.2	11.1 ± 1.6§
CD3⁺ T cells, ×10⁶‡	2.1 ± 0.4	1.3 ± 0.2	86.6 ± 9.6	246 ± 83.3
CD19⁺B220⁺ B cells, %‡	15.3 ± 5.3	9.4 ± 1.2	48.1 ± 7.2	27.2 ± 8.4
CD19⁺B220⁺ B cells, ×10⁶‡	9 ± 3.9	2.1 ± 0.2	117 ± 13.1	505 ± 107§

Frequency (as percentage of total BM or spleen cells) and absolute numbers of cells in various hematopoietic compartments (± SEM). LSK (Lin⁻Sca1⁺cKit⁺); CMP (Lin⁻Sca1⁻cKit⁺CD34⁺Fcγ R^lo); GMP (Lin⁻Sca1⁻cKit⁺CD34⁺Fcγ R^hi); MEPs (Lin⁻Sca1⁻cKit⁺CD34⁻Fcγ R^−/lo); and CLPs (Lin⁻Sca1^locKit^loIL7Rα ⁺).

NC indicates not calculated.

n = 6.

†

n = 5.

‡

n = 4.

P<.05, by unpaired 2-tailed Student t test.

‖

P<.05, by Wilcoxon-Mann-Whitney test.

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