Primer pairs used to screen the entire FPGS gene (accession no. NM_004957)
| Primer . | Position on cDNA . | Length, bp . | Position on gDNA . | Length, bp . |
|---|---|---|---|---|
| EX1-up 5′-CGCGGCATAACGACCCAG-3′ | 108-125 | Exon 1 | ||
| EX3-dw 5′-TTCCCCTTCGTCCCAGTGAC-3′ | 351-370 | 263 | Exon 3 | 1570 |
| EX3-up 5′-CCGGCTGAACATCATCCA-3′ | 332-349 | Exon 3 | ||
| EX10-dw 5′-AGCATCGGACACAGGTATAGA-3′ | 878-898 | 567 | Exon 10 | 4071 |
| EX8-up 5′-TCTCCTCTCTTGGCATCGA-3′ | 712-730 | Exon 8 | ||
| EX13-dw 5′-CGGTCCCCGGTAGCATTGA-3′ | 1288-1306 | 594 | Exon 13 | 2452 |
| EX11-up 5′-CAAAGGCATCCAGGCCAGG-3′ | 1033-1051 | Exon 11 | ||
| EX15-dw 5′-TGCTCTTCGTCCAGGTGGTT-3′ | 1473-1492 | 460 | Exon 15 | 4471 |
| EX15-up 5′-TTCACAGTGACACTGGACCA-3′ | 1416-1435 | Exon 15 | ||
| EX15b-dw 5′-AGTATGTAAGTTCATGGGGAG-3′ | 1862-1882 | 467 | Exon 15 | 467 |
| Primer . | Position on cDNA . | Length, bp . | Position on gDNA . | Length, bp . |
|---|---|---|---|---|
| EX1-up 5′-CGCGGCATAACGACCCAG-3′ | 108-125 | Exon 1 | ||
| EX3-dw 5′-TTCCCCTTCGTCCCAGTGAC-3′ | 351-370 | 263 | Exon 3 | 1570 |
| EX3-up 5′-CCGGCTGAACATCATCCA-3′ | 332-349 | Exon 3 | ||
| EX10-dw 5′-AGCATCGGACACAGGTATAGA-3′ | 878-898 | 567 | Exon 10 | 4071 |
| EX8-up 5′-TCTCCTCTCTTGGCATCGA-3′ | 712-730 | Exon 8 | ||
| EX13-dw 5′-CGGTCCCCGGTAGCATTGA-3′ | 1288-1306 | 594 | Exon 13 | 2452 |
| EX11-up 5′-CAAAGGCATCCAGGCCAGG-3′ | 1033-1051 | Exon 11 | ||
| EX15-dw 5′-TGCTCTTCGTCCAGGTGGTT-3′ | 1473-1492 | 460 | Exon 15 | 4471 |
| EX15-up 5′-TTCACAGTGACACTGGACCA-3′ | 1416-1435 | Exon 15 | ||
| EX15b-dw 5′-AGTATGTAAGTTCATGGGGAG-3′ | 1862-1882 | 467 | Exon 15 | 467 |