TCF mRNA expression in OB lines and primary MSC by RT-PCR
| . | TCF1 . | TCF3 . | TCF4 . | LEF1 . | β-actin . |
|---|---|---|---|---|---|
| C2C12 | ++ | + | +++ | + | ++++ |
| C3H10T1/2 | ++ | + | +++ | − | ++++ |
| MG63 | ++ | + | +++ | − | ++++ |
| FOB1.19 | ++ | + | +++ | + | ++++ |
| hnMSC | + | ++ | +++ | ++ | ++++ |
| mmMSC#32 | ++ | +++ | +++ | ++ | ++++ |
| mmMSC#36 | + | +++ | +++ | ++ | ++++ |
| . | TCF1 . | TCF3 . | TCF4 . | LEF1 . | β-actin . |
|---|---|---|---|---|---|
| C2C12 | ++ | + | +++ | + | ++++ |
| C3H10T1/2 | ++ | + | +++ | − | ++++ |
| MG63 | ++ | + | +++ | − | ++++ |
| FOB1.19 | ++ | + | +++ | + | ++++ |
| hnMSC | + | ++ | +++ | ++ | ++++ |
| mmMSC#32 | ++ | +++ | +++ | ++ | ++++ |
| mmMSC#36 | + | +++ | +++ | ++ | ++++ |
+ indicates a weak fluorescent density; ++, between weak and middle fluorescent density; +++, middle fluorescent density; and ++++, strong fluorescent density of ethidium bromide-stained DNA.