Sequential studies of WT1 mutations in AML patients
| UPN* . | Date . | Status . | Chromosomal abnormality . | WT1 mutation . | Other genetic mutations . | |
|---|---|---|---|---|---|---|
| Amino acid change . | Mutant by gene scan, percentage . | |||||
| 1 | 7/29/1994 | Initial | — | V382fsx385 | 54.02 | FLT3/ITD |
| 2/13/1995 | CR1 | — | — | — | ||
| 3 | 4/9/1999 | Initial | t(7;11)(p15;p15),+8 | G380fsX448 | 39.53 | KRAS |
| 7/9/1999 | CR1 | — | — | — | ||
| 5/3/2000 | Relapse1 | t(7;11)(p15;p15),+8 | G380fsX448 | 35.10 | KRAS | |
| 4 | 10/30/1995 | Initial | — | N381fsX450 | 41.55 | NPM1 |
| 1/15/1996 | CR1 | — | — | — | ||
| 10/22/1996 | Relapse1 | ND | — | 0 | NPM1 | |
| 12/21/1999 | CR2 | — | ||||
| 8/1/2000 | Relapse2 | —7,t(12;18)(p11;q11) | — | 0 | — | |
| 6 | 12/12/1995 | Initial | t(8;21)(q22;q22) | Q380fsX384 | 8.71 | — |
| 11/26/2002 | CR1 | — | — | — | ||
| 7 | 10/8/1996 | Initial | t(7;11)(p15;p15) | V382fsX385 | 59.24 | — |
| 11/8/1996 | CR1 | — | — | — | ||
| 8/22/1997 | Relapse1 | t(7;11)(p15;p15) | V382fsX385 | 40.45 | — | |
| 8 | 4/6/1999 | Initial | t(8;21)(q21;q22) | E409fsX421 | 44.27 | — |
| 7/23/2002 | CR1 | — | — | — | ||
| 10 | 6/30/2000 | Initial | t(4;12;11)(q21;q13;p15),—7 | P465fsX472 | 46.43 | — |
| 9/22/2000 | CR1 | — | — | — | ||
| 11 | 9/1/2000 | Initial | — | F460X | 21.07 | CEBPA |
| 9/22/2000 | CR1 | — | — | — | ||
| 9/24/2001 | Relapse1 | NM | — | 0 | CEBPA | |
| 1/25/2005 | CR2 | — | — | — | ||
| 12 | 3/15/2001 | Initial | 46,XY | R378fsX386 | 47.27 | — |
| 11/16/2001 | CR1 | — | — | — | ||
| 10/8/2002 | Relapse1 | add(19)(q13) | R378fsX386 | 88.36 | FLT3/ITD | |
| 13 | 6/26/2001 | Initial | t(8;21)(q22;q22) | G447V | NA | — |
| 7/31/2001 | CR1 | — | — | — | ||
| 5/10/2002 | Relapse1 | t(8;21)(q22;q22),t(3;12)(p21;q24) | G447V | NA | — | |
| 14 | 4/3/2002 | Initial | — | D379fsX386 | 21.93 | — |
| 5/14/2002 | CR1 | — | — | — | ||
| 16 | 5/29/2003 | Initial | — | Q380fsX384 | 11.9 | FLT3/ITD, NPM1 |
| 7/17/2003 | CR1 | — | — | — | ||
| 12/26/2003 | Relapse1 | ND | Q380fsX384 | 37.76 | FLT3/ITD, NPM1 | |
| 18 | 8/7/2003 | Initial | t(7;11)(p15;p15) | G379fsX381 | 34.16 | KRAS |
| 11/20/2003 | CR1 | — | — | — | ||
| 1/4/2005 | Relapse1 | t(7;11)(p15;p15) | G379fsX381 | 8.65 | — | |
| 19 | 8/17/2007 | Initial | t(7;11)(p15;p15) | V381fsX384 | 47.43 | FLT3/ITD |
| 9/28/2007 | CR1 | — | — | — | ||
| 2/14/2008 | Relapse1 | t(7;11)(p15;p15) | — | 0 | — | |
| 21 | 3/8/2005 | Initial | — | A379fsX383 | 35.63 | FLT3/ITD, NRAS, NPM1 |
| 5/10/2005 | CR1 | — | — | — | ||
| 12/2/2005 | Relapse1 | — | A379fsX383 | 40.46 | FLT3/ITD, NPM1 | |
| S378fsX387 | 39.56 | |||||
| 22 | 2005/5/20 | Initial | −X,+21 | D377fsX384 | 11.71 | CEBPA |
| 6/22/2005 | CR1 | — | — | — | ||
| 5/16/2006 | Relapse1 | −X,+21 | D377fsX384 | 61.55 | CEBPA | |
| 23 | 6/15/2005 | Initial | — | S382fsX388 | 42.20 | NPM1, FLT3/TKD |
| 9/13/2005 | CR1 | — | — | — | ||
| 12/9/2005 | Relapse1 | — | S382fsX388 | 33.32 | NPM1, FLT3/TKD | |
| V382fsX385 | 27.44 | |||||
| 25 | 7/21/2006 | Initial | — | Y378fsX447 | 49.63 | FLT3/TKD, RUNX1 |
| 9/21/2006 | CR1 | — | — | — | ||
| 7/15/2008 | Relapse1 | — | Y378fsX447 | 6.32 | FLT3/TKD | |
| 26 | 10/19/2006 | Initial | NM | R378fsX450 | 53.90 | FLT3/ITD, NRAS |
| 1/30/2007 | CR1 | — | — | — | ||
| 6/5/2007 | Relapse1 | ND | R378fsX450 | 36.44 | FLT3/ITD, NRAS | |
| 11/2/2007 | CR2 | ND | — | — | ||
| 12/3/2007 | Relapse 2 | t(8;11)(q24;p15),del(12)(p13),add(19)(p13), t(2;17)(q21;q25) | — | 0 | FLT3/ITD | |
| 29 | 1/2/2007 | Initial | — | P369fsX384 | 11.38 | MLL/PTD |
| 7/13/2007 | CR1 | — | — | — | ||
| 1/7/2008 | Relapse1 | — | P369fsX384 | 29.56 | MLL/PTD | |
| 30 | 7/9/2007 | Initial | +4, del(9)(q13q22),+10 | Y402X | 47.04 | CEBPA |
| 8/21/2007 | CR1 | — | — | — | ||
| 31 | 9/11/2007 | Initial | — | S381X | NA | FLT3/ITD, MLL/PTD |
| 11/19/2007 | CR1 | — | — | — | ||
| 32 | 12/4/2007 | Initial | — | K399fsX448 | 28.70 | CEBPA |
| 12/27/2007 | CR1 | — | — | — | ||
| 4/1/2008 | Relapse1 | — | K399fsX448 | 7.20 | CEBPA | |
| 33 | 12/8/1996 | Initial | — | — | 0 | |
| 1/21/1997 | CR1 | — | — | — | ||
| 9/11/2001 | CR2 | — | — | — | ||
| 6/10/2002 | Relapse 2 | ND | L381fsX452 | 11.09 | NRAS, NPM1 | |
| 34 | 7/27/2000 | Initial | — | — | 0 | NPM1 |
| 11/10/2000 | CR1 | — | — | — | ||
| 7/17/2001 | Relapse1 | — | D379fsX386 | 20.70 | NPM1 | |
| 10/23/2001 | CR2 | — | — | |||
| 5/7/2002 | Relapse 2 | del(6)(p21) | D379fsX386 | 28.06 | NPM1 | |
| 35 | 4/18/2002 | Initial | — | — | NA | CEBPA |
| 5/13/2002 | CR1 | — | — | — | ||
| 11/19/2002 | Relapse1 | t(1;3)(p36;q21),del(3)(p13p21) | R471T | NA | CEBPA | |
| 12/16/2002 | CR2 | — | — | — | ||
| UPN* . | Date . | Status . | Chromosomal abnormality . | WT1 mutation . | Other genetic mutations . | |
|---|---|---|---|---|---|---|
| Amino acid change . | Mutant by gene scan, percentage . | |||||
| 1 | 7/29/1994 | Initial | — | V382fsx385 | 54.02 | FLT3/ITD |
| 2/13/1995 | CR1 | — | — | — | ||
| 3 | 4/9/1999 | Initial | t(7;11)(p15;p15),+8 | G380fsX448 | 39.53 | KRAS |
| 7/9/1999 | CR1 | — | — | — | ||
| 5/3/2000 | Relapse1 | t(7;11)(p15;p15),+8 | G380fsX448 | 35.10 | KRAS | |
| 4 | 10/30/1995 | Initial | — | N381fsX450 | 41.55 | NPM1 |
| 1/15/1996 | CR1 | — | — | — | ||
| 10/22/1996 | Relapse1 | ND | — | 0 | NPM1 | |
| 12/21/1999 | CR2 | — | ||||
| 8/1/2000 | Relapse2 | —7,t(12;18)(p11;q11) | — | 0 | — | |
| 6 | 12/12/1995 | Initial | t(8;21)(q22;q22) | Q380fsX384 | 8.71 | — |
| 11/26/2002 | CR1 | — | — | — | ||
| 7 | 10/8/1996 | Initial | t(7;11)(p15;p15) | V382fsX385 | 59.24 | — |
| 11/8/1996 | CR1 | — | — | — | ||
| 8/22/1997 | Relapse1 | t(7;11)(p15;p15) | V382fsX385 | 40.45 | — | |
| 8 | 4/6/1999 | Initial | t(8;21)(q21;q22) | E409fsX421 | 44.27 | — |
| 7/23/2002 | CR1 | — | — | — | ||
| 10 | 6/30/2000 | Initial | t(4;12;11)(q21;q13;p15),—7 | P465fsX472 | 46.43 | — |
| 9/22/2000 | CR1 | — | — | — | ||
| 11 | 9/1/2000 | Initial | — | F460X | 21.07 | CEBPA |
| 9/22/2000 | CR1 | — | — | — | ||
| 9/24/2001 | Relapse1 | NM | — | 0 | CEBPA | |
| 1/25/2005 | CR2 | — | — | — | ||
| 12 | 3/15/2001 | Initial | 46,XY | R378fsX386 | 47.27 | — |
| 11/16/2001 | CR1 | — | — | — | ||
| 10/8/2002 | Relapse1 | add(19)(q13) | R378fsX386 | 88.36 | FLT3/ITD | |
| 13 | 6/26/2001 | Initial | t(8;21)(q22;q22) | G447V | NA | — |
| 7/31/2001 | CR1 | — | — | — | ||
| 5/10/2002 | Relapse1 | t(8;21)(q22;q22),t(3;12)(p21;q24) | G447V | NA | — | |
| 14 | 4/3/2002 | Initial | — | D379fsX386 | 21.93 | — |
| 5/14/2002 | CR1 | — | — | — | ||
| 16 | 5/29/2003 | Initial | — | Q380fsX384 | 11.9 | FLT3/ITD, NPM1 |
| 7/17/2003 | CR1 | — | — | — | ||
| 12/26/2003 | Relapse1 | ND | Q380fsX384 | 37.76 | FLT3/ITD, NPM1 | |
| 18 | 8/7/2003 | Initial | t(7;11)(p15;p15) | G379fsX381 | 34.16 | KRAS |
| 11/20/2003 | CR1 | — | — | — | ||
| 1/4/2005 | Relapse1 | t(7;11)(p15;p15) | G379fsX381 | 8.65 | — | |
| 19 | 8/17/2007 | Initial | t(7;11)(p15;p15) | V381fsX384 | 47.43 | FLT3/ITD |
| 9/28/2007 | CR1 | — | — | — | ||
| 2/14/2008 | Relapse1 | t(7;11)(p15;p15) | — | 0 | — | |
| 21 | 3/8/2005 | Initial | — | A379fsX383 | 35.63 | FLT3/ITD, NRAS, NPM1 |
| 5/10/2005 | CR1 | — | — | — | ||
| 12/2/2005 | Relapse1 | — | A379fsX383 | 40.46 | FLT3/ITD, NPM1 | |
| S378fsX387 | 39.56 | |||||
| 22 | 2005/5/20 | Initial | −X,+21 | D377fsX384 | 11.71 | CEBPA |
| 6/22/2005 | CR1 | — | — | — | ||
| 5/16/2006 | Relapse1 | −X,+21 | D377fsX384 | 61.55 | CEBPA | |
| 23 | 6/15/2005 | Initial | — | S382fsX388 | 42.20 | NPM1, FLT3/TKD |
| 9/13/2005 | CR1 | — | — | — | ||
| 12/9/2005 | Relapse1 | — | S382fsX388 | 33.32 | NPM1, FLT3/TKD | |
| V382fsX385 | 27.44 | |||||
| 25 | 7/21/2006 | Initial | — | Y378fsX447 | 49.63 | FLT3/TKD, RUNX1 |
| 9/21/2006 | CR1 | — | — | — | ||
| 7/15/2008 | Relapse1 | — | Y378fsX447 | 6.32 | FLT3/TKD | |
| 26 | 10/19/2006 | Initial | NM | R378fsX450 | 53.90 | FLT3/ITD, NRAS |
| 1/30/2007 | CR1 | — | — | — | ||
| 6/5/2007 | Relapse1 | ND | R378fsX450 | 36.44 | FLT3/ITD, NRAS | |
| 11/2/2007 | CR2 | ND | — | — | ||
| 12/3/2007 | Relapse 2 | t(8;11)(q24;p15),del(12)(p13),add(19)(p13), t(2;17)(q21;q25) | — | 0 | FLT3/ITD | |
| 29 | 1/2/2007 | Initial | — | P369fsX384 | 11.38 | MLL/PTD |
| 7/13/2007 | CR1 | — | — | — | ||
| 1/7/2008 | Relapse1 | — | P369fsX384 | 29.56 | MLL/PTD | |
| 30 | 7/9/2007 | Initial | +4, del(9)(q13q22),+10 | Y402X | 47.04 | CEBPA |
| 8/21/2007 | CR1 | — | — | — | ||
| 31 | 9/11/2007 | Initial | — | S381X | NA | FLT3/ITD, MLL/PTD |
| 11/19/2007 | CR1 | — | — | — | ||
| 32 | 12/4/2007 | Initial | — | K399fsX448 | 28.70 | CEBPA |
| 12/27/2007 | CR1 | — | — | — | ||
| 4/1/2008 | Relapse1 | — | K399fsX448 | 7.20 | CEBPA | |
| 33 | 12/8/1996 | Initial | — | — | 0 | |
| 1/21/1997 | CR1 | — | — | — | ||
| 9/11/2001 | CR2 | — | — | — | ||
| 6/10/2002 | Relapse 2 | ND | L381fsX452 | 11.09 | NRAS, NPM1 | |
| 34 | 7/27/2000 | Initial | — | — | 0 | NPM1 |
| 11/10/2000 | CR1 | — | — | — | ||
| 7/17/2001 | Relapse1 | — | D379fsX386 | 20.70 | NPM1 | |
| 10/23/2001 | CR2 | — | — | |||
| 5/7/2002 | Relapse 2 | del(6)(p21) | D379fsX386 | 28.06 | NPM1 | |
| 35 | 4/18/2002 | Initial | — | — | NA | CEBPA |
| 5/13/2002 | CR1 | — | — | — | ||
| 11/19/2002 | Relapse1 | t(1;3)(p36;q21),del(3)(p13p21) | R471T | NA | CEBPA | |
| 12/16/2002 | CR2 | — | — | — | ||
UPN indicates unique patient number; CR, complete remission; ND, not done; NM, no mitosis; —, negative; and NA, not applicable because the mutations are point mutations that cannot be determined by GeneScan.
Patients 1 through 31 had WT1 mutations at diagnosis; patients 32 through 35 had no WT1 mutations at diagnosis but acquired the mutation at relapse. The data of serial studies in another 107 patients, who did not have WT1 mutation either at diagnosis or at relapse, are not shown in this table.