Quantification of Munc13-4 in mouse platelets
| . | Wild-type . | Unc13dJinx Het . | ||
|---|---|---|---|---|
| ng/5 × 107 platelets* . | Molecules/platelet† . | ng/5 × 107 platelets* . | Molecules/platelet† . | |
| Munc13-4 | 3.31 ± 0.50 | 317.6 ± 47.6 | 2.43 ± 0.15 | 233 ± 14.8 |
| VAMP-8‡ | 8.36 ± 0.11 | 8360 ± 110 | ND | ND |
| Syntaxin 2 | 4.87 ± 0.33 | 1766 ± 121 | ND | ND |
| Syntaxin 4 | 4.61 ± 0.52 | 1625 ± 184 | ND | ND |
| . | Wild-type . | Unc13dJinx Het . | ||
|---|---|---|---|---|
| ng/5 × 107 platelets* . | Molecules/platelet† . | ng/5 × 107 platelets* . | Molecules/platelet† . | |
| Munc13-4 | 3.31 ± 0.50 | 317.6 ± 47.6 | 2.43 ± 0.15 | 233 ± 14.8 |
| VAMP-8‡ | 8.36 ± 0.11 | 8360 ± 110 | ND | ND |
| Syntaxin 2 | 4.87 ± 0.33 | 1766 ± 121 | ND | ND |
| Syntaxin 4 | 4.61 ± 0.52 | 1625 ± 184 | ND | ND |
ND indicates not determined.
Mass of proteins in nanograms (ng) per 5 × 107 platelets was determined by quantitative Western blotting using recombinant proteins to generate a standard curve.
Number of molecules per platelet was calculated using the molecular weight for Munc13-4 (125 kDa), VAMP-8 (12 kDa), syntaxin 2 (33 kDa), and syntaxin 4 (34 kDa).
From Graham et al.3