Change of hematologic compartments and cell populations after Gfi1b deletion
| . | Gfi1bfl/fl . | Gfi1bfl/flMx-Cre tg . | Fold change . | P . |
|---|---|---|---|---|
| Number of BM cells ×106 | 36 ± 9 (n = 28) | 41 ± 13 (n = 27) | 1.13 | .21 |
| Number of splenocytes ×106 | 94 ± 9 (n = 5) | 180 ± 25 (n = 9) | 2 | .04 |
| % Lin− cells in BM | 1.9 ± 0.3 (n = 14) | 3.1 ± 0.4 (n = 14) | 1.63 | .002 |
| Number of Lin− cells ×106 | 0.7 ± 0.1 (n = 14) | 1.5 ± 0.2 (n = 14) | 2 | .01 |
| Number of HSCs in BM | 1000 ± 115 (n = 14) | 39 000 ± 11 000 (n = 14) | 39 | .0001 |
| Number of HSCs in spleen | 442 ± 150 (n = 3) | 51 000 ± 12 800 (n = 5) | 115 | .01 |
| Number of HSCs per 1 mL of blood | 15 ± 13 (n = 6) | 1435 ± 200 (n = 6) | 95 | .01 |
| Number of CD34+ HSC | 1100 ± 500 (n = 3) | 28 000 ± 9000 (n = 3) | 25 | .04 |
| Number of CD34− HSC | 780 ± 280 (n = 3) | 25 500 ± 500 (n = 3) | 32 | .001 |
| . | Gfi1bfl/fl . | Gfi1bfl/flMx-Cre tg . | Fold change . | P . |
|---|---|---|---|---|
| Number of BM cells ×106 | 36 ± 9 (n = 28) | 41 ± 13 (n = 27) | 1.13 | .21 |
| Number of splenocytes ×106 | 94 ± 9 (n = 5) | 180 ± 25 (n = 9) | 2 | .04 |
| % Lin− cells in BM | 1.9 ± 0.3 (n = 14) | 3.1 ± 0.4 (n = 14) | 1.63 | .002 |
| Number of Lin− cells ×106 | 0.7 ± 0.1 (n = 14) | 1.5 ± 0.2 (n = 14) | 2 | .01 |
| Number of HSCs in BM | 1000 ± 115 (n = 14) | 39 000 ± 11 000 (n = 14) | 39 | .0001 |
| Number of HSCs in spleen | 442 ± 150 (n = 3) | 51 000 ± 12 800 (n = 5) | 115 | .01 |
| Number of HSCs per 1 mL of blood | 15 ± 13 (n = 6) | 1435 ± 200 (n = 6) | 95 | .01 |
| Number of CD34+ HSC | 1100 ± 500 (n = 3) | 28 000 ± 9000 (n = 3) | 25 | .04 |
| Number of CD34− HSC | 780 ± 280 (n = 3) | 25 500 ± 500 (n = 3) | 32 | .001 |
The number of bone marrow (BM) cells, splenocytes, and % of Lin− cells was determined in wt and Gfi1b-deficient mice. The increase in number of splenocytes is mostly due to increase of number of erythroid progenitors. HSCs are defined by immunophenotype as Lin−, Sca-1+, Kit+, CD150+, CD48−. Depicted are mean values, SEM, and number of samples. P values are based on unpaired 2-sided t test.