Expression of intracellular signaling molecules in CD11b+/Gr-1+ cells from 3-month doxycycline-treated c-fms/MMP12 mice
| . | BM . | PBMC . | Spleen . | Lung . | ||||
|---|---|---|---|---|---|---|---|---|
| −DOX . | +DOX . | −DOX . | +DOX . | −DOX . | +DOX . | −DOX . | +DOX . | |
| pStat3 | 2.65 ± 1.21 | 11.00 ± 2.58 | 2.28 ± 0.45 | 45.37 ± 6.06 | 1.98 ± 0.18 | 15.29 ± 4.10 | 2.58 ± 1.35 | 12.99 ± 1.29 |
| pP38 | 1.68 ± 0.56 | 14.11 ± 2.32 | 1.75 ± 0.76 | 14.88 ± 4.28 | 1.55 ± 0.20 | 12.21 ± 3.09 | 2.05 ± 0.48 | 9.77 ± 1.23 |
| pErk | 3.71 ± 0.55 | 14.51 ± 3.12 | 2.36 ± 1.83 | 10.64 ± 0.99 | 2.88 ± 0.78 | 7.92 ± 1.34 | 3.97 ± 0.46 | 8.34 ± 0.57 |
| pNFκB | 2.45 ± 0.85 | 5.12 ± 1.64 | 2.05 ± 0.29 | 32.16 ± 8.58 | 1.63 ± 0.47 | 9.36 ± 2.00 | 1.76 ± 0.57 | 7.18 ± 1.68 |
| . | BM . | PBMC . | Spleen . | Lung . | ||||
|---|---|---|---|---|---|---|---|---|
| −DOX . | +DOX . | −DOX . | +DOX . | −DOX . | +DOX . | −DOX . | +DOX . | |
| pStat3 | 2.65 ± 1.21 | 11.00 ± 2.58 | 2.28 ± 0.45 | 45.37 ± 6.06 | 1.98 ± 0.18 | 15.29 ± 4.10 | 2.58 ± 1.35 | 12.99 ± 1.29 |
| pP38 | 1.68 ± 0.56 | 14.11 ± 2.32 | 1.75 ± 0.76 | 14.88 ± 4.28 | 1.55 ± 0.20 | 12.21 ± 3.09 | 2.05 ± 0.48 | 9.77 ± 1.23 |
| pErk | 3.71 ± 0.55 | 14.51 ± 3.12 | 2.36 ± 1.83 | 10.64 ± 0.99 | 2.88 ± 0.78 | 7.92 ± 1.34 | 3.97 ± 0.46 | 8.34 ± 0.57 |
| pNFκB | 2.45 ± 0.85 | 5.12 ± 1.64 | 2.05 ± 0.29 | 32.16 ± 8.58 | 1.63 ± 0.47 | 9.36 ± 2.00 | 1.76 ± 0.57 | 7.18 ± 1.68 |
Cells from the BM, blood, spleen, and lung of doxycycline-treated or untreated bitransgenic mice were stained with CD11b and GR-1 Abs and followed by the intracellular staining with pStat3, pErk, pP38, or pNFκB Ab. Percentage numbers of different cells represent intracellular-stained positive cells in CD11b+/Gr-1+ cells from 4 independent experiments (n = 4).