Vβ spectratype analysis shows oligoclonal expansion of TCR-transduced allogeneic T cells in contrast to syngeneic TCR-transduced T cells after early AT
| Vβ . | Day 0 (AT) . | Days 40-44 . | |||||
|---|---|---|---|---|---|---|---|
| Syngeneic . | Allogeneic . | ||||||
| 1 | — | — | — | — | — | — | — |
| 2 | — | — | — | — | — | 136 | 136 |
| 3 | — | — | 157 | — | — | — | 148, 154 |
| 4 | — | — | — | — | — | — | — |
| 5.1 | — | — | — | — | — | — | — |
| 5.2 | 139 | 139 | 139 | 139 | 139 | 139 | 139 |
| 6 | — | 135 | — | — | — | — | — |
| 7 | — | — | — | — | — | 152 | 143 |
| 8.1 | — | — | — | — | — | — | — |
| 8.2 | — | — | — | — | 145 | — | — |
| 8.3 | — | — | — | — | 132 | — | 150 |
| 9 | — | — | — | — | — | — | — |
| 10 | — | — | — | — | 169 | 163 | 151 |
| 11 | — | — | — | — | — | — | — |
| 12 | — | — | — | — | — | — | 168 |
| 13 | — | 162 | — | — | — | — | 168 |
| 14 | — | — | — | — | 155 | 155 | — |
| 15 | — | 141 | — | — | 138 | — | — |
| 16 | — | — | — | — | 167 | 159 | 156 |
| 17 | 144 | — | 144 | — | 165 | — | 135 |
| 18 | — | — | — | — | — | — | — |
| Vβ . | Day 0 (AT) . | Days 40-44 . | |||||
|---|---|---|---|---|---|---|---|
| Syngeneic . | Allogeneic . | ||||||
| 1 | — | — | — | — | — | — | — |
| 2 | — | — | — | — | — | 136 | 136 |
| 3 | — | — | 157 | — | — | — | 148, 154 |
| 4 | — | — | — | — | — | — | — |
| 5.1 | — | — | — | — | — | — | — |
| 5.2 | 139 | 139 | 139 | 139 | 139 | 139 | 139 |
| 6 | — | 135 | — | — | — | — | — |
| 7 | — | — | — | — | — | 152 | 143 |
| 8.1 | — | — | — | — | — | — | — |
| 8.2 | — | — | — | — | 145 | — | — |
| 8.3 | — | — | — | — | 132 | — | 150 |
| 9 | — | — | — | — | — | — | — |
| 10 | — | — | — | — | 169 | 163 | 151 |
| 11 | — | — | — | — | — | — | — |
| 12 | — | — | — | — | — | — | 168 |
| 13 | — | 162 | — | — | — | — | 168 |
| 14 | — | — | — | — | 155 | 155 | — |
| 15 | — | 141 | — | — | 138 | — | — |
| 16 | — | — | — | — | 167 | 159 | 156 |
| 17 | 144 | — | 144 | — | 165 | — | 135 |
| 18 | — | — | — | — | — | — | — |
A total of 40 × 106 TCR gene-transduced B10.A T cells were adoptively transferred early (21 days after HCT) into established allogeneic (B10.A → B6) or syngeneic recipients (B10A → B10A). Mice were killed 40 days after AT. Splenocytes were sorted for CD8+GFP+ T cells, and CDR3-size spectratype analysis was performed as described in “TCR spectratype analysis and assessment of proliferation.” TCR-engineered T cells directly from the culture were used as controls (AT). CDR3-size length skewing was determined by comparison of the experimental spectratypes to the respective naive B10.A controls (n = 6) as described in “TCR spectratype analysis and assessment of proliferation.” Skewed CDR3-size lengths of individual allogeneic (n = 3) and syngeneic (n = 3) mice are shown.
— indicates no skewing.