PD-L1 blockade does not induce oligoclonal expansion of allogeneic TCR-transduced T cells after late AT
| Vβ . | Allogeneic . | |||||
|---|---|---|---|---|---|---|
| Without PD-L1 . | With PD-L1 . | |||||
| 1 | — | — | — | — | — | — |
| 2 | — | — | — | — | — | — |
| 3 | — | — | — | — | — | — |
| 4 | — | — | — | — | 148 | — |
| 5.1 | — | — | — | — | — | — |
| 5.2 | 139 | 139 | 139 | 139 | 139 | NA |
| 6 | — | — | — | — | — | — |
| 7 | 146 | — | — | — | — | — |
| 8.1 | — | — | — | — | — | — |
| 8.2 | — | — | — | — | — | — |
| 8.3 | 138 | — | 141 | — | — | — |
| 9 | — | — | — | — | — | — |
| 10 | — | — | — | — | — | — |
| 11 | — | — | — | — | — | — |
| 12 | — | — | — | — | — | — |
| 13 | — | 162 | 162 | 165 | — | — |
| 14 | — | — | — | — | — | — |
| 15 | — | — | — | — | — | — |
| 16 | — | 169 | — | — | — | — |
| 17 | — | — | — | 140 | — | — |
| 18 | — | — | — | — | — | — |
| Vβ . | Allogeneic . | |||||
|---|---|---|---|---|---|---|
| Without PD-L1 . | With PD-L1 . | |||||
| 1 | — | — | — | — | — | — |
| 2 | — | — | — | — | — | — |
| 3 | — | — | — | — | — | — |
| 4 | — | — | — | — | 148 | — |
| 5.1 | — | — | — | — | — | — |
| 5.2 | 139 | 139 | 139 | 139 | 139 | NA |
| 6 | — | — | — | — | — | — |
| 7 | 146 | — | — | — | — | — |
| 8.1 | — | — | — | — | — | — |
| 8.2 | — | — | — | — | — | — |
| 8.3 | 138 | — | 141 | — | — | — |
| 9 | — | — | — | — | — | — |
| 10 | — | — | — | — | — | — |
| 11 | — | — | — | — | — | — |
| 12 | — | — | — | — | — | — |
| 13 | — | 162 | 162 | 165 | — | — |
| 14 | — | — | — | — | — | — |
| 15 | — | — | — | — | — | — |
| 16 | — | 169 | — | — | — | — |
| 17 | — | — | — | 140 | — | — |
| 18 | — | — | — | — | — | — |
A total of 40 × 106 TCR gene-transduced allogeneic (B10.A) T cells were adoptively transferred late (56 days after HCT) into established allogeneic (B10.A → B6) recipients. Treatment with α-PD-L1 or isotype control was performed as described in the legend of Figure 6. Forty days after AT, CD8+GFP+ T cells were sorted from spleens, and CDR3-size spectratype analysis was performed as described in “TCR spectratype analysis and assessment of proliferation.” Skewed CDR3-size lengths of individual mice after treatment with α-PD-L1 (n = 3) or isotype control (n = 3) are shown.
— indicates no skewing; and NA, not available.