Primer sequences used for qRT-PCR
| Gene symbol . | GenBank accession no. . | Primer set sequence (5′-> 3′) . | Amplicon size, bp . |
|---|---|---|---|
| Beta-actin (h) | NM_001101 | Forward: ATCGTGCGTGACATTAAGGAGAAG Reverse: AGGAAGGAAGGCTGGAAGAGTG | 179 |
| BCL-2 (h) | NM_000633 | Forward: TTCAACACAGACCCACCCAGAG Reverse: GCAGGATAGCAGCACAGGATTG | 276 |
| BCL-XL (h) | NM_138578 | Forward: GCAGCCGAGAGCCGAAAGG Reverse: GGATGTGGTGGAGCAGAGAAGG | 161 |
| MCL-1 (h) | NM_021960 | Forward: AAGAGGCTGGGATGGGTTTGTG Reverse: TTGGTGGTGGTGGTGGTTGG | 178 |
| ATF3 (h) | NM_001674 | Forward: AGCAGCAGAGAACCATCAAGGC Reverse: GAAAGGCGGGCAGGACACC | 184 |
| ATF4 (h) | NM_001675 | Forward: TCCGAATGGCTGGCTGTGG Reverse: AGTGTAGTCTGGCTTCCTATCTCC | 420 |
| ATF6 (h) | NM_007348 | Forward: AGTTCGTCAGTTCCTCCTTACCTC Reverse: AGTTCTCTGCCTGCCACCAAG | 226 |
| CHOP (h) | NM_004083 | Forward: TGCTTCTCTGGCTTGGCTGAC Reverse: CCGTTTCCTGGTTCTCCCTTGG | 145 |
| GRP-78 (h) | NM_005347 | Forward: AGGAGGAGGACAAGAAGGAGGAC Reverse: CAGGAGTGAAGGCGACATAGGAC | 156 |
| Beta-actin (m) | NM_007393 | Forward: GCGACAGCAGTTGGTTGGAG Reverse: TTTGGGAGGGTGAGGGACTTC | 165 |
| Bcl-2 (m) | NM_177410 | Forward: CTGGTTGAATGAGTCTGGGCTTTG Reverse: AGTGTTGGAGGTCTGGTGCTTAC | 337 |
| Bcl-xl (m) | NM_009743 | Forward: ACTGTGGCTGGTGTGGTTCTG Reverse: AGTGGTTCTCCTGGTAGCAATGG | 139 |
| Mcl-1 (m) | NM_008562 | Forward: GCGTGTTATGCTCCCAGTTCC Reverse: TGCCAATCCAAGAATGCCAATCC | 240 |
| Atf3 (m) | NM_007498 | Forward: GTGGAGCAGGCAGGAGCATC Reverse: CTTGGAAGGGCGTTGTCAGC | 204 |
| Atf4 (m) | NM_009716 | Forward: GCCTGACTCTGCTGCTTACATTAC Reverse: CCCTTGCCTTACGGACCTCTTC | 452 |
| Atf6 (m) | NM_001081304 | Forward: CAGAGGCAGCACACGCATTC Reverse: GGTCAGCAGGAGCAGAGAAGG | 279 |
| Chop (m) | NM_007837 | Forward: CATCTTCATACACCACCACACCTG Reverse: CTCGTTCTCCTGCTCCTTCTCC | 432 |
| Grp-78 (m) | NM_022310 | Forward: GTCTGCTTCGTGTCTCCTCCTG Reverse: TCCTCCTTCTTGTCCTCCTCCTC | 220 |
| Gene symbol . | GenBank accession no. . | Primer set sequence (5′-> 3′) . | Amplicon size, bp . |
|---|---|---|---|
| Beta-actin (h) | NM_001101 | Forward: ATCGTGCGTGACATTAAGGAGAAG Reverse: AGGAAGGAAGGCTGGAAGAGTG | 179 |
| BCL-2 (h) | NM_000633 | Forward: TTCAACACAGACCCACCCAGAG Reverse: GCAGGATAGCAGCACAGGATTG | 276 |
| BCL-XL (h) | NM_138578 | Forward: GCAGCCGAGAGCCGAAAGG Reverse: GGATGTGGTGGAGCAGAGAAGG | 161 |
| MCL-1 (h) | NM_021960 | Forward: AAGAGGCTGGGATGGGTTTGTG Reverse: TTGGTGGTGGTGGTGGTTGG | 178 |
| ATF3 (h) | NM_001674 | Forward: AGCAGCAGAGAACCATCAAGGC Reverse: GAAAGGCGGGCAGGACACC | 184 |
| ATF4 (h) | NM_001675 | Forward: TCCGAATGGCTGGCTGTGG Reverse: AGTGTAGTCTGGCTTCCTATCTCC | 420 |
| ATF6 (h) | NM_007348 | Forward: AGTTCGTCAGTTCCTCCTTACCTC Reverse: AGTTCTCTGCCTGCCACCAAG | 226 |
| CHOP (h) | NM_004083 | Forward: TGCTTCTCTGGCTTGGCTGAC Reverse: CCGTTTCCTGGTTCTCCCTTGG | 145 |
| GRP-78 (h) | NM_005347 | Forward: AGGAGGAGGACAAGAAGGAGGAC Reverse: CAGGAGTGAAGGCGACATAGGAC | 156 |
| Beta-actin (m) | NM_007393 | Forward: GCGACAGCAGTTGGTTGGAG Reverse: TTTGGGAGGGTGAGGGACTTC | 165 |
| Bcl-2 (m) | NM_177410 | Forward: CTGGTTGAATGAGTCTGGGCTTTG Reverse: AGTGTTGGAGGTCTGGTGCTTAC | 337 |
| Bcl-xl (m) | NM_009743 | Forward: ACTGTGGCTGGTGTGGTTCTG Reverse: AGTGGTTCTCCTGGTAGCAATGG | 139 |
| Mcl-1 (m) | NM_008562 | Forward: GCGTGTTATGCTCCCAGTTCC Reverse: TGCCAATCCAAGAATGCCAATCC | 240 |
| Atf3 (m) | NM_007498 | Forward: GTGGAGCAGGCAGGAGCATC Reverse: CTTGGAAGGGCGTTGTCAGC | 204 |
| Atf4 (m) | NM_009716 | Forward: GCCTGACTCTGCTGCTTACATTAC Reverse: CCCTTGCCTTACGGACCTCTTC | 452 |
| Atf6 (m) | NM_001081304 | Forward: CAGAGGCAGCACACGCATTC Reverse: GGTCAGCAGGAGCAGAGAAGG | 279 |
| Chop (m) | NM_007837 | Forward: CATCTTCATACACCACCACACCTG Reverse: CTCGTTCTCCTGCTCCTTCTCC | 432 |
| Grp-78 (m) | NM_022310 | Forward: GTCTGCTTCGTGTCTCCTCCTG Reverse: TCCTCCTTCTTGTCCTCCTCCTC | 220 |
h indicates human; and m, mouse.