Table 1 Binding of protein S to... | American Society of Hematology

Table 1

Binding of protein S to domain-specific monoclonal antibodies and enhancement of APC-mediated cleavage of FVa at Arg306 by protein S

	WT PS	Chimera I	Chimera II	Chimera III
MK 21 (Gla)	1.93 ± 0.78	1.67 ± 0.33	1.62 ± 0.78	1.92 ± 0.83
MK 41 (EGF3-EGF4)	0.76 ± 0.18	1.00 ± 0.32	0.50 ± 0.26	0.75 ± 0.46
MK 54 (EGF1)	0.25 ± 0.13	0.21 ± 0.12	0.17 ± 0.06	0.20 ± 0.07
MK 67 (TSR)	0.22 ± 0.09	0.18 ± 0.03	0.13 ± 0.06	0.15 ± 0.09
EC₅₀ for FVa^R506Q/R679Q inactivation	5.18 ± 0.36	5.20 ± 1.19	6.31 ± 0.53	4.92 ± 0.95

	WT PS	Chimera I	Chimera II	Chimera III
MK 21 (Gla)	1.93 ± 0.78	1.67 ± 0.33	1.62 ± 0.78	1.92 ± 0.83
MK 41 (EGF3-EGF4)	0.76 ± 0.18	1.00 ± 0.32	0.50 ± 0.26	0.75 ± 0.46
MK 54 (EGF1)	0.25 ± 0.13	0.21 ± 0.12	0.17 ± 0.06	0.20 ± 0.07
MK 67 (TSR)	0.22 ± 0.09	0.18 ± 0.03	0.13 ± 0.06	0.15 ± 0.09
EC₅₀ for FVa^R506Q/R679Q inactivation	5.18 ± 0.36	5.20 ± 1.19	6.31 ± 0.53	4.92 ± 0.95

PS, protein S.

K_{d app}/EC₅₀ values obtained from 3 independent experiments are given in nanomolars and are expressed as mean ± SD.