List of primers used
| . | Primer direction . | Primer name . | Primer sequence . |
|---|---|---|---|
| 1 | F | 18S forward | GTAACCCGTTGAACCCCATT |
| 2 | R | 18S reverse | CCATCCAATCGGTAGTAGCG |
| 3 | F | gapdhF | TGCACCACCAACTGCTTAGC |
| 4 | R | gapdhR | GGCATGGACTGTGGTCATGAG |
| 5 | F | ahr F | ACATCACCTACGCCAGTCGC |
| 6 | R | ahr R | TCTATGCCGCTTGGAAGGAT |
| 7 | F | mir-29b-1 ahr chip site 1F (140bp) | GATGCTGTTCTCTTTGGGCTGTC |
| 8 | R | mir-29b-1 ahr chip site 1R (140bp) | AAGCCACTCGGGGAGGAGAA |
| 9 | F | mir-29b-1 ahr chip site 2-F (145BP) | CAGAGGGTGCATGGATCATAGGGT |
| 10 | R | mir-29b-1 ahr chip site 2-R (145bp) | GCAGCAACCTCTGAGATGGCAG |
| 11 | F | F-real time cyp1a1 | GTCTTGGACCTCTTTGGAGCT |
| 12 | R | R-real time cyp1a1 | GTGACCTGCCAATCACTGTG |
| 13 | F | TBET REAL TIME F | CAGAATGCCGAGATTACTCAG |
| 14 | R | TBET REAL TIME R | AGGATACTGGTTGGGTAGGA |
| 15 | F | EOMES REAL TIME F | AACAACACCCAGATGATAGTC |
| 16 | R | EOMES REAL TIME R | TCATAGTTGTCTCTGAAG |
| 17 | F | mir-29b promoter cloning F kpnI | ATATATGGTACCAGGGTTAATGCTCCAACC |
| 18 | R | mir-29b promoter cloning R hindIII | ATATATAAGCTTTGTAGTTAGCGACCTCTGCT |
| 19 | F | cyp1a1 promoter F kpnI | ATATATGGTACCGCTGTCAGCTGTGTTCCCTTC |
| 20 | R | cyp1a1 promoter R hindIII | ATATATAAGCTTTGGGACCATGGGCAAGTCC |
| 21 | F | IFNg F rtpcr | CCAGGACCCATATGTAAAAG |
| 22 | R | IFNg R rtpcr | TGGCTCTGCATTATTTTTC |
| 23 | F | mir-29-up prom-A sub F | GAGAAACCAAATTAAAATTAGAAAAATCTGGTCAACAGTGATCACGT |
| 24 | R | mir-29b-up prom-A sub R | ACGTGATCACTGTTGACCAGATTTTTCTAATTTTAATTTGGTTTCTC |
| 25 | F | mir-29b-up prom-del sub F | GAGAAACCAAATTAAAATTAGTCTGGTCAACAGTGATCACGT |
| 26 | R | mir-29b-up prom-del sub R | ACGTGATCACTGTTGACCAGACTAATTTTAATTTGGTTTCTC |
| 27 | F | mir-29b-down prom-A sub F | TTAAGAGGAAATTGGTCACTAAAAATCATCTCGAGTGGTGGTGATTC |
| 28 | R | mir-29b-down prom-A sub R | GAATCACCACCACTCGAGATGATTTTTAGTGACCAATTTCCTCTTAA |
| 29 | F | mir-29b-down prom-del sub F | TTAAGAGGAAATTGGTCACTTCATCTCGAGTGGTGGTGATTC |
| 30 | R | mir-29b-down prom-del sub R | GAATCACCACCACTCGAGATGAAGTGACCAATTTCCTCTTAA |
| 31 | F | mir-29bc pgl3 F cloning | ATATATGGTACCTGACACGCAAGGTGGCACG |
| 32 | R | mir-29bc pgl3 R cloning | ATATATAAGCTTTAGGAAGAGGCTTCAACTGGGC |
| . | Primer direction . | Primer name . | Primer sequence . |
|---|---|---|---|
| 1 | F | 18S forward | GTAACCCGTTGAACCCCATT |
| 2 | R | 18S reverse | CCATCCAATCGGTAGTAGCG |
| 3 | F | gapdhF | TGCACCACCAACTGCTTAGC |
| 4 | R | gapdhR | GGCATGGACTGTGGTCATGAG |
| 5 | F | ahr F | ACATCACCTACGCCAGTCGC |
| 6 | R | ahr R | TCTATGCCGCTTGGAAGGAT |
| 7 | F | mir-29b-1 ahr chip site 1F (140bp) | GATGCTGTTCTCTTTGGGCTGTC |
| 8 | R | mir-29b-1 ahr chip site 1R (140bp) | AAGCCACTCGGGGAGGAGAA |
| 9 | F | mir-29b-1 ahr chip site 2-F (145BP) | CAGAGGGTGCATGGATCATAGGGT |
| 10 | R | mir-29b-1 ahr chip site 2-R (145bp) | GCAGCAACCTCTGAGATGGCAG |
| 11 | F | F-real time cyp1a1 | GTCTTGGACCTCTTTGGAGCT |
| 12 | R | R-real time cyp1a1 | GTGACCTGCCAATCACTGTG |
| 13 | F | TBET REAL TIME F | CAGAATGCCGAGATTACTCAG |
| 14 | R | TBET REAL TIME R | AGGATACTGGTTGGGTAGGA |
| 15 | F | EOMES REAL TIME F | AACAACACCCAGATGATAGTC |
| 16 | R | EOMES REAL TIME R | TCATAGTTGTCTCTGAAG |
| 17 | F | mir-29b promoter cloning F kpnI | ATATATGGTACCAGGGTTAATGCTCCAACC |
| 18 | R | mir-29b promoter cloning R hindIII | ATATATAAGCTTTGTAGTTAGCGACCTCTGCT |
| 19 | F | cyp1a1 promoter F kpnI | ATATATGGTACCGCTGTCAGCTGTGTTCCCTTC |
| 20 | R | cyp1a1 promoter R hindIII | ATATATAAGCTTTGGGACCATGGGCAAGTCC |
| 21 | F | IFNg F rtpcr | CCAGGACCCATATGTAAAAG |
| 22 | R | IFNg R rtpcr | TGGCTCTGCATTATTTTTC |
| 23 | F | mir-29-up prom-A sub F | GAGAAACCAAATTAAAATTAGAAAAATCTGGTCAACAGTGATCACGT |
| 24 | R | mir-29b-up prom-A sub R | ACGTGATCACTGTTGACCAGATTTTTCTAATTTTAATTTGGTTTCTC |
| 25 | F | mir-29b-up prom-del sub F | GAGAAACCAAATTAAAATTAGTCTGGTCAACAGTGATCACGT |
| 26 | R | mir-29b-up prom-del sub R | ACGTGATCACTGTTGACCAGACTAATTTTAATTTGGTTTCTC |
| 27 | F | mir-29b-down prom-A sub F | TTAAGAGGAAATTGGTCACTAAAAATCATCTCGAGTGGTGGTGATTC |
| 28 | R | mir-29b-down prom-A sub R | GAATCACCACCACTCGAGATGATTTTTAGTGACCAATTTCCTCTTAA |
| 29 | F | mir-29b-down prom-del sub F | TTAAGAGGAAATTGGTCACTTCATCTCGAGTGGTGGTGATTC |
| 30 | R | mir-29b-down prom-del sub R | GAATCACCACCACTCGAGATGAAGTGACCAATTTCCTCTTAA |
| 31 | F | mir-29bc pgl3 F cloning | ATATATGGTACCTGACACGCAAGGTGGCACG |
| 32 | R | mir-29bc pgl3 R cloning | ATATATAAGCTTTAGGAAGAGGCTTCAACTGGGC |