Expression of CD20 and CD74 as determined by hA20 and hLL1, respectively, in cell lines used in the study
| . | Mean fluorescence intensity . | ||
|---|---|---|---|
| FITC control . | CD20 . | CD74 . | |
| JeKo-1 | 4 | 161 | 11 |
| Mino | 3 | 697 | 98 |
| Granta-519 | 3 | 1734 | 53 |
| Daudi | 6 | 483 | 60 |
| Raji | 4 | 960 | 310 |
| RPMI8226 | 5 | 25 | 10 |
| KMS-12PE | 4 | 4 | 23 |
| KMS-12BM | 4 | 590 | 58 |
| KMS-11 | 7 | 17 | 96 |
| CAG | 5 | 4 | 139 |
| . | Mean fluorescence intensity . | ||
|---|---|---|---|
| FITC control . | CD20 . | CD74 . | |
| JeKo-1 | 4 | 161 | 11 |
| Mino | 3 | 697 | 98 |
| Granta-519 | 3 | 1734 | 53 |
| Daudi | 6 | 483 | 60 |
| Raji | 4 | 960 | 310 |
| RPMI8226 | 5 | 25 | 10 |
| KMS-12PE | 4 | 4 | 23 |
| KMS-12BM | 4 | 590 | 58 |
| KMS-11 | 7 | 17 | 96 |
| CAG | 5 | 4 | 139 |
Ag expression analysis was done by indirect immunofluorescence staining. Cells were incubated with 10 μg/mL amounts of hA20 and hLL1 for CD20 and CD74 expression analysis, respectively, for 45 minutes followed by FITC-conjugated F(ab')2 fragment goat anti-human Fc-specific secondary antibody. All incubations were done on ice followed by washing and flow cytometric analysis.