Production of IL-6 by HDMECs following incubation with P falciparum components
| Stimulant . | IL-6, pg/mL . | SEM . | n . |
|---|---|---|---|
| Experiment group 1 | |||
| Control | 0 | 0 | 6 |
| Pf-GPI, 1 μg/mL | 0 | 0 | 4 |
| IFN-γ, 20 ng/mL | 38.01 | 13.01 | 6 |
| IFN-γ + Pf-GPI | 1312.42 | 628.27 | 6 |
| IFN-γ + PAM3Cys4, 10 μg/mL* | > 20 000 | — | 3 |
| Experiment group 2 | |||
| Control | 0 | 0 | 11 |
| Pf sonicate, 1×107/cm2 | 27.92 | 7.57 | 11 |
| IFN-γ, 20 ng/mL | 0 | 0 | 4 |
| IFN-γ + Pf sonicate | 0 | 0 | 4 |
| Experiment group 3 | |||
| Control | 21.75 | 3.02 | 4 |
| Merozoite, 0.5 μL/cm | 51.38 | 10.47 | 4 |
| Experiment group 4 | |||
| Control | 0 | 0 | 4 |
| Pf food vacuole, 0.1 μL/cm2 | > 20 000 | — | 4 |
| Purified Pf hemozoin, 20 μg/cm2 | 0 | 0 | 4 |
| Stimulant . | IL-6, pg/mL . | SEM . | n . |
|---|---|---|---|
| Experiment group 1 | |||
| Control | 0 | 0 | 6 |
| Pf-GPI, 1 μg/mL | 0 | 0 | 4 |
| IFN-γ, 20 ng/mL | 38.01 | 13.01 | 6 |
| IFN-γ + Pf-GPI | 1312.42 | 628.27 | 6 |
| IFN-γ + PAM3Cys4, 10 μg/mL* | > 20 000 | — | 3 |
| Experiment group 2 | |||
| Control | 0 | 0 | 11 |
| Pf sonicate, 1×107/cm2 | 27.92 | 7.57 | 11 |
| IFN-γ, 20 ng/mL | 0 | 0 | 4 |
| IFN-γ + Pf sonicate | 0 | 0 | 4 |
| Experiment group 3 | |||
| Control | 21.75 | 3.02 | 4 |
| Merozoite, 0.5 μL/cm | 51.38 | 10.47 | 4 |
| Experiment group 4 | |||
| Control | 0 | 0 | 4 |
| Pf food vacuole, 0.1 μL/cm2 | > 20 000 | — | 4 |
| Purified Pf hemozoin, 20 μg/cm2 | 0 | 0 | 4 |
All HDMECs were incubated for 24 hours with the indicated components.
— indicates not applicable.
PAM3Cys4 is a synthetic TLR-2 ligand.