Effect of acute plasma on endothelial permeability
| Study group . | n . | Plasma sample, h following admission . | Dilution . | Cytokine levels in plasma samples, pg/mL . | HDMEC permeability following incubation with plasma for 24 h . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| IL-6 . | IL-10 . | IL-1β . | IFN-γ . | TNF-α . | % Permeability . | % Change in TER . | ||||
| Severe malaria, median (IQR) | 17 | 0 | 1:10 | 450 (95-2950) | 1984 (1309-11641) | 0 (0-0) | 36 (21-139) | 0 (0-8) | 1.72† (1.55-2.0) | −8.92‡ (−14.64-(−)6.80) |
| Correlation to % permeability, r (P)* | 17 | 0 | 1:10 | 0.519 (.033) | 0.534 (.027) | −0.160 (.540) | −0.040 (.877) | 0.662(.004) | — | — |
| Severe malaria, median (IQR) | 11 | 48 | 1:10 | 100 (62.5-252.5) | 531 (387-1069) | ND | ND | ND | 1.47 (1.34-1.65) | -11.7† (−15.04-(−)2.43) |
| Correlation to % permeability, r (P)* | 11 | 48 | 1:10 | −0.151 (.658) | −0.4840 (.131) | — | — | — | — | — |
| Control, median (IQR) | 6 | — | 1:10 | ND | ND | ND | ND | ND | 1.40 (1.2-1.5) | 5.5 (1.5-7.2) |
| Severe malaria, median (IQR) | 8 | 48 | 1:5 | 83 (51-175) | 521 (339-718) | ND | ND | ND | 1.61† (1.43-2.33) | −13.2† (−28.5-(−)1.76) |
| Control, median (IQR) | 7 | — | 1:5 | ND | ND | ND | ND | ND | 1.24 (1.17-1.39) | 7.92 (8.43-14.3) |
| Study group . | n . | Plasma sample, h following admission . | Dilution . | Cytokine levels in plasma samples, pg/mL . | HDMEC permeability following incubation with plasma for 24 h . | |||||
|---|---|---|---|---|---|---|---|---|---|---|
| IL-6 . | IL-10 . | IL-1β . | IFN-γ . | TNF-α . | % Permeability . | % Change in TER . | ||||
| Severe malaria, median (IQR) | 17 | 0 | 1:10 | 450 (95-2950) | 1984 (1309-11641) | 0 (0-0) | 36 (21-139) | 0 (0-8) | 1.72† (1.55-2.0) | −8.92‡ (−14.64-(−)6.80) |
| Correlation to % permeability, r (P)* | 17 | 0 | 1:10 | 0.519 (.033) | 0.534 (.027) | −0.160 (.540) | −0.040 (.877) | 0.662(.004) | — | — |
| Severe malaria, median (IQR) | 11 | 48 | 1:10 | 100 (62.5-252.5) | 531 (387-1069) | ND | ND | ND | 1.47 (1.34-1.65) | -11.7† (−15.04-(−)2.43) |
| Correlation to % permeability, r (P)* | 11 | 48 | 1:10 | −0.151 (.658) | −0.4840 (.131) | — | — | — | — | — |
| Control, median (IQR) | 6 | — | 1:10 | ND | ND | ND | ND | ND | 1.40 (1.2-1.5) | 5.5 (1.5-7.2) |
| Severe malaria, median (IQR) | 8 | 48 | 1:5 | 83 (51-175) | 521 (339-718) | ND | ND | ND | 1.61† (1.43-2.33) | −13.2† (−28.5-(−)1.76) |
| Control, median (IQR) | 7 | — | 1:5 | ND | ND | ND | ND | ND | 1.24 (1.17-1.39) | 7.92 (8.43-14.3) |
IQR indicates interquartile range; —, not applicable; and ND, not done.
Correlations between percentage permeability and each cytokine (r) were performed by Spearman rank sum test. P values (P) for each comparison are given in parentheses.
P < .05 compared with controls by Mann-Whitney U test.
P < .01 compared with controls by Mann-Whitney U test.