In vivo reconstitution capacity of CD34+ACE+ and CD34+ACE− sorted cells and transplantation in secondary NOD/SCID recipients
| Age, wk . | No. of specimens . | Fetal liver . | BM . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CD34+ACE+ . | CD34+ACE− . | CD34+ACE+ . | CD34+ACE− . | ||||||||
| No. of injected cells . | Engrafted mice* . | Secondary transplantation . | No. of injected cells . | Engrafted mice* . | No. of injected cells . | Engrafted mice* . | Secondary transplantation . | No. of injected cells . | Engrafted mice* . | ||
| 14 | 1 | 1 × 103 | 2/3 | ND | 1.5 × 103 | 0/2 | ND | ||||
| 2 × 103 | 3/3 | ND | ND | ||||||||
| 5 × 103 | 3/3 | 1/2 | ND | ||||||||
| 10 × 103 | 3/3 | 2/2 | ND | ||||||||
| 16 | 1 | 17 × 103 | 1/1 | 1/1 | 42 × 103 | 0/1 | ND | ||||
| 2 | 40 × 103 | 1/1 | ND | 94 × 103 | 0/1 | ND | |||||
| 3 | 9 × 103 | 1/1 | ND | 6 × 103 | 0/1 | ND | |||||
| 18 | 1 | 0.5 × 103 | 1/2 | ND | 5 × 103 | 0/2 | ND | ||||
| 2 × 103 | 1/2 | ND | ND | ||||||||
| 5 × 103 | 1/2 | ND | ND | ||||||||
| 19 | 1 | ND | 1.7 × 103 | 2/2 | ND | 7 × 103 | 0/2 | ||||
| 20 | 1 | ND | 17 × 103 | 1/1 | 0/2 | 35 × 103 | 0/1 | ||||
| 22 | 1 | ND | 1.4 × 103 | 1/1 | ND | 2.5 × 103 | 0/1 | ||||
| Age, wk . | No. of specimens . | Fetal liver . | BM . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| CD34+ACE+ . | CD34+ACE− . | CD34+ACE+ . | CD34+ACE− . | ||||||||
| No. of injected cells . | Engrafted mice* . | Secondary transplantation . | No. of injected cells . | Engrafted mice* . | No. of injected cells . | Engrafted mice* . | Secondary transplantation . | No. of injected cells . | Engrafted mice* . | ||
| 14 | 1 | 1 × 103 | 2/3 | ND | 1.5 × 103 | 0/2 | ND | ||||
| 2 × 103 | 3/3 | ND | ND | ||||||||
| 5 × 103 | 3/3 | 1/2 | ND | ||||||||
| 10 × 103 | 3/3 | 2/2 | ND | ||||||||
| 16 | 1 | 17 × 103 | 1/1 | 1/1 | 42 × 103 | 0/1 | ND | ||||
| 2 | 40 × 103 | 1/1 | ND | 94 × 103 | 0/1 | ND | |||||
| 3 | 9 × 103 | 1/1 | ND | 6 × 103 | 0/1 | ND | |||||
| 18 | 1 | 0.5 × 103 | 1/2 | ND | 5 × 103 | 0/2 | ND | ||||
| 2 × 103 | 1/2 | ND | ND | ||||||||
| 5 × 103 | 1/2 | ND | ND | ||||||||
| 19 | 1 | ND | 1.7 × 103 | 2/2 | ND | 7 × 103 | 0/2 | ||||
| 20 | 1 | ND | 17 × 103 | 1/1 | 0/2 | 35 × 103 | 0/1 | ||||
| 22 | 1 | ND | 1.4 × 103 | 1/1 | ND | 2.5 × 103 | 0/1 | ||||
ND indicates not done.
Engraftment is based on flow cytometric analysis of BM cells from recipient mice harvested at 4-6 months after transplantation and is defined as at least 1% of human CD45+ cells.