Genomic, transcriptional, and phenotypic analysis of the KIR repertoire in EUs
| EU . | HLA-C . | HLA-B . | KIR2DS1/KIR2DL1 . | KIR2DS2/2DL2/2DL3 . | KIR3DS1/KIR3DL1 . | KIR2DS4 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype, 2DS1/2DL1 . | Transcripts, 2DS1/2DL1 (ratio) . | Phenotype, % EB6+ . | Genotype, 2DS2/2DL2/2DL3 . | Transcripts, 2DS2/2DL2/2DL3 . | Phenotype, % GL183+ . | Genotype, 3DS1/3DL1 . | Transcripts, 3DS1/3DL1 (ratio) . | Phenotype, % Z27.3.7+ . | Genotype, 2DS4 . | Transcripts, 2DS4/1D . | Phenotype, % FES172+ . | |||
| Individual values for each EU | ||||||||||||||
| 1 | C1 | Bw4 Bw6 | −/+ | −/+ | 15 | −/−/+ | −/−/+ | 24 | −/+ | −/+ | 30 | + | +/− | 53 |
| 2 | C1 | Bw4 Bw6 | −/+ | −/+ | 21 | −/−/+ | −/−/+ | 57* | −/+ | −/+ | 7 | + | +/− | 81 |
| 3 | C1 | Bw4 Bw6 | ND | −/+ | ND | ND | −/−/+ | ND | ND | −/+ | ND | ND | +/− | ND |
| 4 | C1 | Bw4 Bw6 | −/+ | −/+ | 6 | +/+/+ | +/+/+ | 63* | −/+ | −/+ | 13 | + | −/+ | 38 |
| 5 | C1 | Bw6 | −/+ | −/+ | 32 | −/−/+ | −/−/+ | 68* | −/+ | −/− | 0 | + | −/− | 0 |
| 6 | C1 | Bw6 | +/+ | +/+ (2.8) | 29 | −/−/+ | −/−/+ | 26 | +/+ | +/+ (28)* | 6 | + | −/+ | 0 |
| 7 | C1 | Bw6 | +/+ | +/+ (61) | 4 | +/+/+ | +/+/+ | ND | +/+ | +/+ (59)* | 1 | + | −/+ | 0 |
| 8 | C1 | Bw6 | ND | +/+ (0.9) | 8 | ND | +/+/+ | 33 | ND | −/+ | 22 | ND | +/+ | 45 |
| 9 | C1 | Bw6 | ND | +/+ (3.7) | ND | ND | −/−/+ | ND | ND | +/+(1.3) | ND | ND | −/+ | ND |
| 10 | C1 C2 | Bw4 Bw6 | +/+ | +/− | 13 | +/+/+ | +/+/+ | 67* | +/+ | +/− | 6 | + | +/+ | 24 |
| 11 | C1 C2 | Bw4 Bw6 | ND | +/+ (4.0) | 50 | ND | −/−/+ | 54* | ND | +/+ (3.0) | 27 | ND | +/+ | 44 |
| 12 | C1 C2 | Bw4 Bw6 | +/+ | +/+ (1.0) | 83 | +/+/+ | +/+/+ | 6 | +/+ | +/+ (1.9) | 6 | + | +/− | 37 |
| 13 | C1 C2 | Bw4 Bw6 | +/+ | +/+ (2.3) | 21 | −/−/+ | −/−/+ | 36 | +/+ | +/+ (0.9) | 26 | + | −/+ | 0 |
| 14 | C1 C2 | Bw4 Bw6 | ND | −/+ | ND | ND | −/−/+ | ND | ND | −/− | ND | ND | +/+ | ND |
| 15 | C1 C2 | Bw6 | −/+ | −/− | 10 | +/+/+ | −/+/+ | 41 | +/+ | +/− | ND | + | +/+ | 16 |
| 16 | C1 C2 | Bw6 | +/+ | +/+ (1.0) | 38 | −/−/+ | −/−/+ | 32 | +/+ | +/+ (28)* | 8 | + | −/+ | 0 |
| 17 | C1 C2 | Bw6 | −/+ | −/+ | 9 | +/+/+ | +/+/+ | 12 | −/+ | −/+ | 5 | + | +/− | 30 |
| 18 | C1 C2 | Bw6 | +/+ | +/+ (0.1) | 47 | −/−/+ | −/−/+ | 63* | −/+ | −/+ | 17 | + | +/+ | 22 |
| 19 | C2 | Bw4 Bw6 | ND | −/+ | ND | ND | −/−/+ | 31 | ND | −/+ | ND | ND | +/− | ND |
| 20 | C2 | Bw6 | −/+ | −/+ | ND | −/−/+ | −/−/+ | ND | −/+ | −/+ | ND | + | −/+ | ND |
| 21 | ND | ND | ND | +/+ (1.0) | 25 | ND | −/−/+ | 54* | ND | +/+ (8.1) | 10 | ND | +/− | 40 |
| 22 | ND | ND | ND | +/+ (3.45) | 31 | ND | +/+/+ | 46 | ND | +/+ (3.8) | 21 | ND | +/− | 45 |
| 23 | ND | ND | ND | −/+ | 5 | ND | +/+/+ | 19 | ND | −/+ | 1 | ND | −/+ | 0 |
| 24 | ND | ND | ND | +/+ (47) | 20 | ND | +/+/+ | 40 | ND | ND | 8 | ND | −/− | ND |
| 25 | ND | ND | ND | −/+ | ND | ND | −/−/+ | 30 | ND | −/+ | ND | ND | −/+ | ND |
| Summary of values by group, median (25th-75th percentile ranges) | ||||||||||||||
| HIV | — | — | — | 1.4 (1.4-1.4) | 11.3 (7.6-22.5) | — | — | 28.4 (19.5-44.1) | — | 2.1 (2.1-2.1) | 11.3 (6.3-15.7) | — | — | 33.7 (18.1-37.7) |
| Total EUs | — | — | — | 1.7 (0.5-3.6) | 15 (9-31) | — | — | 36 (23-56.7) | — | 4.7 (1.5-28.0) | 8.6 (6.9-17.5) | — | — | 30.5 (20.9-42.0) |
| C | — | — | — | 4.1 (3.1-4.8) | 14.5 (7-26) | — | — | 35.2 (20-48.3) | — | 3.0 (2.0-10.0) | 12 (8.8-21.5) | — | — | 32.6 (21.7-65.0) |
| EU . | HLA-C . | HLA-B . | KIR2DS1/KIR2DL1 . | KIR2DS2/2DL2/2DL3 . | KIR3DS1/KIR3DL1 . | KIR2DS4 . | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Genotype, 2DS1/2DL1 . | Transcripts, 2DS1/2DL1 (ratio) . | Phenotype, % EB6+ . | Genotype, 2DS2/2DL2/2DL3 . | Transcripts, 2DS2/2DL2/2DL3 . | Phenotype, % GL183+ . | Genotype, 3DS1/3DL1 . | Transcripts, 3DS1/3DL1 (ratio) . | Phenotype, % Z27.3.7+ . | Genotype, 2DS4 . | Transcripts, 2DS4/1D . | Phenotype, % FES172+ . | |||
| Individual values for each EU | ||||||||||||||
| 1 | C1 | Bw4 Bw6 | −/+ | −/+ | 15 | −/−/+ | −/−/+ | 24 | −/+ | −/+ | 30 | + | +/− | 53 |
| 2 | C1 | Bw4 Bw6 | −/+ | −/+ | 21 | −/−/+ | −/−/+ | 57* | −/+ | −/+ | 7 | + | +/− | 81 |
| 3 | C1 | Bw4 Bw6 | ND | −/+ | ND | ND | −/−/+ | ND | ND | −/+ | ND | ND | +/− | ND |
| 4 | C1 | Bw4 Bw6 | −/+ | −/+ | 6 | +/+/+ | +/+/+ | 63* | −/+ | −/+ | 13 | + | −/+ | 38 |
| 5 | C1 | Bw6 | −/+ | −/+ | 32 | −/−/+ | −/−/+ | 68* | −/+ | −/− | 0 | + | −/− | 0 |
| 6 | C1 | Bw6 | +/+ | +/+ (2.8) | 29 | −/−/+ | −/−/+ | 26 | +/+ | +/+ (28)* | 6 | + | −/+ | 0 |
| 7 | C1 | Bw6 | +/+ | +/+ (61) | 4 | +/+/+ | +/+/+ | ND | +/+ | +/+ (59)* | 1 | + | −/+ | 0 |
| 8 | C1 | Bw6 | ND | +/+ (0.9) | 8 | ND | +/+/+ | 33 | ND | −/+ | 22 | ND | +/+ | 45 |
| 9 | C1 | Bw6 | ND | +/+ (3.7) | ND | ND | −/−/+ | ND | ND | +/+(1.3) | ND | ND | −/+ | ND |
| 10 | C1 C2 | Bw4 Bw6 | +/+ | +/− | 13 | +/+/+ | +/+/+ | 67* | +/+ | +/− | 6 | + | +/+ | 24 |
| 11 | C1 C2 | Bw4 Bw6 | ND | +/+ (4.0) | 50 | ND | −/−/+ | 54* | ND | +/+ (3.0) | 27 | ND | +/+ | 44 |
| 12 | C1 C2 | Bw4 Bw6 | +/+ | +/+ (1.0) | 83 | +/+/+ | +/+/+ | 6 | +/+ | +/+ (1.9) | 6 | + | +/− | 37 |
| 13 | C1 C2 | Bw4 Bw6 | +/+ | +/+ (2.3) | 21 | −/−/+ | −/−/+ | 36 | +/+ | +/+ (0.9) | 26 | + | −/+ | 0 |
| 14 | C1 C2 | Bw4 Bw6 | ND | −/+ | ND | ND | −/−/+ | ND | ND | −/− | ND | ND | +/+ | ND |
| 15 | C1 C2 | Bw6 | −/+ | −/− | 10 | +/+/+ | −/+/+ | 41 | +/+ | +/− | ND | + | +/+ | 16 |
| 16 | C1 C2 | Bw6 | +/+ | +/+ (1.0) | 38 | −/−/+ | −/−/+ | 32 | +/+ | +/+ (28)* | 8 | + | −/+ | 0 |
| 17 | C1 C2 | Bw6 | −/+ | −/+ | 9 | +/+/+ | +/+/+ | 12 | −/+ | −/+ | 5 | + | +/− | 30 |
| 18 | C1 C2 | Bw6 | +/+ | +/+ (0.1) | 47 | −/−/+ | −/−/+ | 63* | −/+ | −/+ | 17 | + | +/+ | 22 |
| 19 | C2 | Bw4 Bw6 | ND | −/+ | ND | ND | −/−/+ | 31 | ND | −/+ | ND | ND | +/− | ND |
| 20 | C2 | Bw6 | −/+ | −/+ | ND | −/−/+ | −/−/+ | ND | −/+ | −/+ | ND | + | −/+ | ND |
| 21 | ND | ND | ND | +/+ (1.0) | 25 | ND | −/−/+ | 54* | ND | +/+ (8.1) | 10 | ND | +/− | 40 |
| 22 | ND | ND | ND | +/+ (3.45) | 31 | ND | +/+/+ | 46 | ND | +/+ (3.8) | 21 | ND | +/− | 45 |
| 23 | ND | ND | ND | −/+ | 5 | ND | +/+/+ | 19 | ND | −/+ | 1 | ND | −/+ | 0 |
| 24 | ND | ND | ND | +/+ (47) | 20 | ND | +/+/+ | 40 | ND | ND | 8 | ND | −/− | ND |
| 25 | ND | ND | ND | −/+ | ND | ND | −/−/+ | 30 | ND | −/+ | ND | ND | −/+ | ND |
| Summary of values by group, median (25th-75th percentile ranges) | ||||||||||||||
| HIV | — | — | — | 1.4 (1.4-1.4) | 11.3 (7.6-22.5) | — | — | 28.4 (19.5-44.1) | — | 2.1 (2.1-2.1) | 11.3 (6.3-15.7) | — | — | 33.7 (18.1-37.7) |
| Total EUs | — | — | — | 1.7 (0.5-3.6) | 15 (9-31) | — | — | 36 (23-56.7) | — | 4.7 (1.5-28.0) | 8.6 (6.9-17.5) | — | — | 30.5 (20.9-42.0) |
| C | — | — | — | 4.1 (3.1-4.8) | 14.5 (7-26) | — | — | 35.2 (20-48.3) | — | 3.0 (2.0-10.0) | 12 (8.8-21.5) | — | — | 32.6 (21.7-65.0) |
HLA-B and -C typing was performed and grouped as HLA-C1 (Asn80), HLA-C2 (Lys80), and Bw4/Bw6 groups, according to KIR recognition pattern. Real-time PCR evaluation of each KIR transcript level was performed in 25 exposed uninfected individuals (EUs) and associated to KIR genotyping in 14 EUs. When KIR transcripts were detected (+), the ratio between activating and inhibitory counterparts was indicated (in parentheses). Phenotypic expression of KIR receptors is indicated as the percentage of KIR+ cells among CD3−CD56+ NK cells using EB6+, GL183+, Z27.3.7+, and FES172† mAbs. Data from total EUs were analyzed in reference to median values and 25th to 75th percentiles (in parentheses) in low-risk Vietnamese unexposed control blood donors (C) and HIV-infected individuals (HIV).
ND indicates not determined; —, not applicable.
Values over the 75th percentile observed in control donors.