Summary of predicted motifs in dominant GPIIIa peptides for binding to HLA-DR molecules and responsiveness of PBMC from AITP patients
| Dominant peptide no. . | Position on GPIIIa* . | HLA-DR molecules bound with high affinity† . | Patients with AITP with PBMC response to peptide . | |
|---|---|---|---|---|
| High-affinity DR expressed . | No high-affinity DR expressed . | |||
| 2 (aa6-20) | PSI domain | None | None | AITP1, AITP3, AITP9, AITP10, AITP11, AITP18, AITP19, AITP20, AITP21,AITP22, AITP24 |
| 44 (aa331-345) | Spanning βA and hybrid domain | DR04, DR07 | AITP8, AITP24 | AITP1, AITP3, AITP10, AITP18, AITP21, |
| 47 (aa361-375) | Hybrid domain | None | None | AITP7, AITP9, AITP10, AITP11, AITP16, AITP19, AITP20, AITP21, AITP22, AITP23, AITP24, AITP26 |
| 53 (aa421-435) | Spanning hybrid and PSI domain | DR04, DR13 | AITP11, AITP-12, AITP18, AITP20 | AITP1, AITP8, AITP9, AITP10, AITP22, AITP24 |
| 70 (aa591-605) | EGF-like domain | None | None | AITP2, AITP7, AITP15, AITP20, AITP24 |
| 77 (aa661-675) | EGF-like domain | DR04, DR15 | AITP11, AITP16, AITP22 | AITP2, AITP8 |
| 82 (aa711-725) | βTD domain (transmembrane/cytoplasmic) | DR01, DR08, DR11, DR13, DR15 | ‡AITP1, AITP3, AITP4, AITP5, AITP9, AITP10, AITP11, AITP16, AITP18, AITP19, AITP20, AITP21, AITP22, AITP24 | ‡AITP7, AITP12 |
| Dominant peptide no. . | Position on GPIIIa* . | HLA-DR molecules bound with high affinity† . | Patients with AITP with PBMC response to peptide . | |
|---|---|---|---|---|
| High-affinity DR expressed . | No high-affinity DR expressed . | |||
| 2 (aa6-20) | PSI domain | None | None | AITP1, AITP3, AITP9, AITP10, AITP11, AITP18, AITP19, AITP20, AITP21,AITP22, AITP24 |
| 44 (aa331-345) | Spanning βA and hybrid domain | DR04, DR07 | AITP8, AITP24 | AITP1, AITP3, AITP10, AITP18, AITP21, |
| 47 (aa361-375) | Hybrid domain | None | None | AITP7, AITP9, AITP10, AITP11, AITP16, AITP19, AITP20, AITP21, AITP22, AITP23, AITP24, AITP26 |
| 53 (aa421-435) | Spanning hybrid and PSI domain | DR04, DR13 | AITP11, AITP-12, AITP18, AITP20 | AITP1, AITP8, AITP9, AITP10, AITP22, AITP24 |
| 70 (aa591-605) | EGF-like domain | None | None | AITP2, AITP7, AITP15, AITP20, AITP24 |
| 77 (aa661-675) | EGF-like domain | DR04, DR15 | AITP11, AITP16, AITP22 | AITP2, AITP8 |
| 82 (aa711-725) | βTD domain (transmembrane/cytoplasmic) | DR01, DR08, DR11, DR13, DR15 | ‡AITP1, AITP3, AITP4, AITP5, AITP9, AITP10, AITP11, AITP16, AITP18, AITP19, AITP20, AITP21, AITP22, AITP24 | ‡AITP7, AITP12 |
From structural analysis of β3 integrin.43
Predicted using the Propred algorithm (http://www.imtech.res.in/raghava/propred/).38,39
Significant association between response to peptide 82 and expression of HLA-DR molecules to which peptide predicted to bind with high affinity (chi-square test = 10; P < .05).