Table 1 List of primers used Primer name... | American Society of Hematology

Table 1

List of primers used

Primer name	Sequence
Genomic PCR*
F1	5′-CACAGTAAGGGTACGTGGAG-3′
R1	5′-TGGAGACAGTGCAGTAGAGC-3′
R2	5′-AGGGTCACAAACAGCCATAC-3′
Cre 3 forward	5′-TTGCCTGCATTACCGGTCGATGCA-3′
Cre 5 reverse	5′-TCGGATCCGCCGCATAACCAG-3′
Quantitative real-time PCR†
Hamp forward	5′-GGGGGATATCAGGCCTCTGCACAGCAGAACAGAAGG-3′
Hamp reverse	5′-GGGGGATATCAGGCCTCTATGTTTTGCAACAGATACC-3′
Dcytb forward	5′-CTCTTCGGAACAGTGATTGCGA-3′
Dcytb reverse	5′-GGGTGTTTCAGGACAAAGAACAG-3′
Ferroportin forward	5′-TGTCAGCCTGCTGTTTGCAGGA-3′
Ferroportin reverse	5′-TCTTGCAGCAACTGTGTCACCG-3′
Dmt1 forward	5′-GTGGTGGCTGCAGTGGTTACGG-3′
Dmt1 reverse	5′-TTGCCACCGCTGGTATCTTCGC-3′
Gapdh forward	5′-GTGGAGATTGTTGCCATCAACGA-3′
Gapdh reverse	5′-CCCATTCTCGGCCTTGACTGT-3′

Primer name	Sequence
Genomic PCR*
F1	5′-CACAGTAAGGGTACGTGGAG-3′
R1	5′-TGGAGACAGTGCAGTAGAGC-3′
R2	5′-AGGGTCACAAACAGCCATAC-3′
Cre 3 forward	5′-TTGCCTGCATTACCGGTCGATGCA-3′
Cre 5 reverse	5′-TCGGATCCGCCGCATAACCAG-3′
Quantitative real-time PCR†
Hamp forward	5′-GGGGGATATCAGGCCTCTGCACAGCAGAACAGAAGG-3′
Hamp reverse	5′-GGGGGATATCAGGCCTCTATGTTTTGCAACAGATACC-3′
Dcytb forward	5′-CTCTTCGGAACAGTGATTGCGA-3′
Dcytb reverse	5′-GGGTGTTTCAGGACAAAGAACAG-3′
Ferroportin forward	5′-TGTCAGCCTGCTGTTTGCAGGA-3′
Ferroportin reverse	5′-TCTTGCAGCAACTGTGTCACCG-3′
Dmt1 forward	5′-GTGGTGGCTGCAGTGGTTACGG-3′
Dmt1 reverse	5′-TTGCCACCGCTGGTATCTTCGC-3′
Gapdh forward	5′-GTGGAGATTGTTGCCATCAACGA-3′
Gapdh reverse	5′-CCCATTCTCGGCCTTGACTGT-3′

Primers used for genotyping and establishment of Hfe recombination efficiency.

Primers used for gene expression analysis by quantitative real-time PCR.