Comparative miRNA expression in CML and normal CD34+ cells
CML . | 17-3p . | 17-5p . | 18a . | 19a . | 19b . | 20 . | 92 . | 106a . | 106b . | 212 . | 142-3p . | 142-5p . | pri-miR-17-92 . | c-myc . |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CP | ||||||||||||||
CP 1 | 3 | 6 | 3 | 3 | 2 | 2 | 2 | 5 | 2 | 7 | NC | 2 | 2 | 3 |
CP 2 | 2 | 5 | 2 | NC | NC | NC | 2 | 5 | NC | 7 | * | * | * | * |
CP 3 | 2 | 4 | 2 | 3 | 2 | 3 | NC | 3 | NC | 3 | −3 | NC | 7 | 9 |
CP 4 | 2 | 4 | 4 | 2 | 2 | 3 | 3 | 2 | 2 | 6 | 2 | 5 | 7 | 3 |
CP 5 | 2 | 5 | 3 | 2 | 2 | NC | 3 | 5 | 2 | 5 | −4 | 2 | 7 | 2 |
CP 6 | NC | 4 | 2 | NC | 2 | 2 | 2 | 4 | NC | 2 | −2 | 2 | NC | 2 |
CP 7 | 3 | 7 | 4 | 2 | NC | 2 | 3 | 8 | NC | 17 | −5 | 2 | 6 | NC |
CP 8 | 2 | 6 | 3 | 2 | NC | 2 | 2 | 5 | NC | 3 | −2 | 2 | 22 | 5 |
CP 9 | NC | 6 | 6 | 5 | 2 | 2 | 2 | 6 | NC | 7 | NC | 3 | 5 | 6 |
CP 10 | 3 | 4 | 3 | 2 | 2 | 2 | 3 | 3 | NC | 2 | NC | 2 | 5 | 2 |
CP 11 | 2 | 3 | 3 | 2 | 2 | 4 | 2 | 3 | NC | 5 | NC | 2 | 6 | 2 |
CP 12 | NC | 2 | 2 | NC | NC | 2 | NC | 2 | NC | 4 | NC | 2 | 58 | * |
CP 13 | 3 | 3 | 4 | 3 | 3 | 2 | 2 | 3 | NC | NC | * | * | 45 | * |
CP 14 | 2 | 4 | 3 | 2 | 2 | 2 | 2 | 5 | NC | * | * | * | 2 | * |
CP 15 | NC | 2 | 2 | 2 | 2 | NC | 2 | 2 | NC | 3 | −5 | NC | 23 | 4 |
CP 16 | NC | 5 | 3 | NC | NC | 2 | 7 | 3 | NC | 11 | 5 | 42 | * | * |
CP 17 | NC | 3 | 2 | 6 | 4 | 2 | 3 | 3 | 2 | 4 | NC | 3 | 31 | 6 |
CP 18 | 14 | 6 | 4 | 2 | 2 | 3 | 19 | 6 | 4 | 79 | * | * | 16 | 4 |
CP 19 | 10 | 4 | 2 | 2 | 3 | 4 | 16 | 6 | 2 | 54 | −3 | NC | 2 | NC |
CP 20 | NC | 4 | 3 | 2 | NC | 2 | 2 | 6 | NC | 4 | 3 | 4 | 2 | NC |
CP 21 | 2 | 3 | 3 | 2 | 2 | 3 | 2 | 6 | NC | 6 | NC | 3 | 18 | 2 |
CP 22 | NC | 4 | 3 | 3 | 2 | 2 | 2 | 6 | NC | 4 | 2 | 3 | NC | NC |
CP 23 | NC | 4 | 3 | 2 | 2 | 2 | 2 | 3 | 2 | 3 | 2 | 2 | NC | 2 |
CP 24 | 6 | 45 | 2 | 2 | 2 | 2 | 6 | 2 | 2 | 17 | −2 | NC | 3 | 2 |
Total | 2.8 | 6 | 3 | 2.3 | 1.9 | 2.2 | 3.8 | 4.3 | 1.4 | 11 | −0.3 | 4.3 | 12.3 | 3.1 |
BC | ||||||||||||||
BC 1 | −2 | NC | −3 | −2 | NC | NC | NC | NC | −3 | NC | −6 | NC | 3 | 2 |
BC 2 | NC | NC | NC | NC | NC | NC | NC | 2 | NC | 4 | NC | 2 | 2 | −2 |
BC 3 | NC | 2 | NC | 2 | NC | 2 | 2 | 2 | NC | 2 | NC | 2 | 5 | NC |
BC 4 | 2 | NC | NC | −2 | NC | −2 | 3 | NC | NC | 31 | −2 | 2 | 5 | −2 |
BC 5 | NC | 2 | NC | NC | NC | NC | NC | 2 | NC | 2 | NC | 2 | 3 | 2 |
BC 6 | 2 | 3 | 2 | NC | 2 | 2 | 2 | 2 | NC | 3 | NC | 3 | 3 | 2 |
BC 7 | NC | NC | NC | NC | NC | NC | NC | NC | NC | 2 | 2 | 4 | 3 | 2 |
Total | 0.9 | 1.5 | 0.6 | 0.3 | 1.1 | 0.9 | 1.6 | 1.6 | 0.4 | 6.4 | −0.3 | 2.3 | 3.4 | 0.7 |
CML . | 17-3p . | 17-5p . | 18a . | 19a . | 19b . | 20 . | 92 . | 106a . | 106b . | 212 . | 142-3p . | 142-5p . | pri-miR-17-92 . | c-myc . |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
CP | ||||||||||||||
CP 1 | 3 | 6 | 3 | 3 | 2 | 2 | 2 | 5 | 2 | 7 | NC | 2 | 2 | 3 |
CP 2 | 2 | 5 | 2 | NC | NC | NC | 2 | 5 | NC | 7 | * | * | * | * |
CP 3 | 2 | 4 | 2 | 3 | 2 | 3 | NC | 3 | NC | 3 | −3 | NC | 7 | 9 |
CP 4 | 2 | 4 | 4 | 2 | 2 | 3 | 3 | 2 | 2 | 6 | 2 | 5 | 7 | 3 |
CP 5 | 2 | 5 | 3 | 2 | 2 | NC | 3 | 5 | 2 | 5 | −4 | 2 | 7 | 2 |
CP 6 | NC | 4 | 2 | NC | 2 | 2 | 2 | 4 | NC | 2 | −2 | 2 | NC | 2 |
CP 7 | 3 | 7 | 4 | 2 | NC | 2 | 3 | 8 | NC | 17 | −5 | 2 | 6 | NC |
CP 8 | 2 | 6 | 3 | 2 | NC | 2 | 2 | 5 | NC | 3 | −2 | 2 | 22 | 5 |
CP 9 | NC | 6 | 6 | 5 | 2 | 2 | 2 | 6 | NC | 7 | NC | 3 | 5 | 6 |
CP 10 | 3 | 4 | 3 | 2 | 2 | 2 | 3 | 3 | NC | 2 | NC | 2 | 5 | 2 |
CP 11 | 2 | 3 | 3 | 2 | 2 | 4 | 2 | 3 | NC | 5 | NC | 2 | 6 | 2 |
CP 12 | NC | 2 | 2 | NC | NC | 2 | NC | 2 | NC | 4 | NC | 2 | 58 | * |
CP 13 | 3 | 3 | 4 | 3 | 3 | 2 | 2 | 3 | NC | NC | * | * | 45 | * |
CP 14 | 2 | 4 | 3 | 2 | 2 | 2 | 2 | 5 | NC | * | * | * | 2 | * |
CP 15 | NC | 2 | 2 | 2 | 2 | NC | 2 | 2 | NC | 3 | −5 | NC | 23 | 4 |
CP 16 | NC | 5 | 3 | NC | NC | 2 | 7 | 3 | NC | 11 | 5 | 42 | * | * |
CP 17 | NC | 3 | 2 | 6 | 4 | 2 | 3 | 3 | 2 | 4 | NC | 3 | 31 | 6 |
CP 18 | 14 | 6 | 4 | 2 | 2 | 3 | 19 | 6 | 4 | 79 | * | * | 16 | 4 |
CP 19 | 10 | 4 | 2 | 2 | 3 | 4 | 16 | 6 | 2 | 54 | −3 | NC | 2 | NC |
CP 20 | NC | 4 | 3 | 2 | NC | 2 | 2 | 6 | NC | 4 | 3 | 4 | 2 | NC |
CP 21 | 2 | 3 | 3 | 2 | 2 | 3 | 2 | 6 | NC | 6 | NC | 3 | 18 | 2 |
CP 22 | NC | 4 | 3 | 3 | 2 | 2 | 2 | 6 | NC | 4 | 2 | 3 | NC | NC |
CP 23 | NC | 4 | 3 | 2 | 2 | 2 | 2 | 3 | 2 | 3 | 2 | 2 | NC | 2 |
CP 24 | 6 | 45 | 2 | 2 | 2 | 2 | 6 | 2 | 2 | 17 | −2 | NC | 3 | 2 |
Total | 2.8 | 6 | 3 | 2.3 | 1.9 | 2.2 | 3.8 | 4.3 | 1.4 | 11 | −0.3 | 4.3 | 12.3 | 3.1 |
BC | ||||||||||||||
BC 1 | −2 | NC | −3 | −2 | NC | NC | NC | NC | −3 | NC | −6 | NC | 3 | 2 |
BC 2 | NC | NC | NC | NC | NC | NC | NC | 2 | NC | 4 | NC | 2 | 2 | −2 |
BC 3 | NC | 2 | NC | 2 | NC | 2 | 2 | 2 | NC | 2 | NC | 2 | 5 | NC |
BC 4 | 2 | NC | NC | −2 | NC | −2 | 3 | NC | NC | 31 | −2 | 2 | 5 | −2 |
BC 5 | NC | 2 | NC | NC | NC | NC | NC | 2 | NC | 2 | NC | 2 | 3 | 2 |
BC 6 | 2 | 3 | 2 | NC | 2 | 2 | 2 | 2 | NC | 3 | NC | 3 | 3 | 2 |
BC 7 | NC | NC | NC | NC | NC | NC | NC | NC | NC | 2 | 2 | 4 | 3 | 2 |
Total | 0.9 | 1.5 | 0.6 | 0.3 | 1.1 | 0.9 | 1.6 | 1.6 | 0.4 | 6.4 | −0.3 | 2.3 | 3.4 | 0.7 |
Numbers indicate the relative fold change rounded up (≥ .6) or down (< .6), respectively as compared with normal CD34+ cells.
NC indicates no change.