Effect of rGel or rGel/BLyS on the protein synthesis
| Patient no. . | % untreated control (dpm/107 cells) . | Protein synthesis, % of control . | |||||
|---|---|---|---|---|---|---|---|
| rGel . | rGel/BLyS . | ||||||
| 1.0 μM . | 2.5 μM . | 5.0 μM . | 1.0 nM . | 2.5 nM . | 5.0 nM . | ||
| 1 | 100 (15 389) | 99 | 77 | 70 | ND | ND | ND |
| 2 | 100 (39 741) | 96 | 109 | 104 | 146 | 104 | 50 |
| 3 | 100 (39 820) | 81 | 73 | 45 | 114 | 69 | 68 |
| 4 | 100 (39 214) | 80 | 79 | 66 | 103 | 90 | 32 |
| 5 | 100 (42 125) | 146 | 97 | 123 | 32 | 28 | 23 |
| 6 | 100 (129 449) | 114 | 110 | 125 | 70 | 51 | 22 |
| 7 | 100 (173 473) | 103 | 68 | 55 | 73 | 49 | 26 |
| Patient no. . | % untreated control (dpm/107 cells) . | Protein synthesis, % of control . | |||||
|---|---|---|---|---|---|---|---|
| rGel . | rGel/BLyS . | ||||||
| 1.0 μM . | 2.5 μM . | 5.0 μM . | 1.0 nM . | 2.5 nM . | 5.0 nM . | ||
| 1 | 100 (15 389) | 99 | 77 | 70 | ND | ND | ND |
| 2 | 100 (39 741) | 96 | 109 | 104 | 146 | 104 | 50 |
| 3 | 100 (39 820) | 81 | 73 | 45 | 114 | 69 | 68 |
| 4 | 100 (39 214) | 80 | 79 | 66 | 103 | 90 | 32 |
| 5 | 100 (42 125) | 146 | 97 | 123 | 32 | 28 | 23 |
| 6 | 100 (129 449) | 114 | 110 | 125 | 70 | 51 | 22 |
| 7 | 100 (173 473) | 103 | 68 | 55 | 73 | 49 | 26 |
Primary CLL cells from patients 1 to 7 were isolated and incubated with either 1.0, 2.5, and 5.0 μM rGel or 1.0, 2.5, and 5.0 nM rGel/BLyS for 72 hours and pulsed with [3H]leucine for 1 hour. At the end of incubation, the leucine incorporation into protein was measured. The values are expressed as percentage of control (untreated) cells.