Effect of rGel or rGel/BLyS on the RNA synthesis
| Patient no. . | % untreated control (dpm/107 cells) . | RNA synthesis, % of control . | |||||
|---|---|---|---|---|---|---|---|
| rGel . | rGel/BlyS . | ||||||
| 1.0 μM . | 2.5 μM . | 5.0 μM . | 1.0 nM . | 2.5 nM . | 5.0 nM . | ||
| 1 | 100 (72 419) | 126 | 106 | 110 | ND | ND | ND |
| 2 | 100 (173 833) | 99 | 99 | 101 | 130 | 29 | 72 |
| 3 | 100 (116 060) | 125 | 99 | 59 | 69 | 79 | 57 |
| 4 | 100 (188 671) | 104 | 88 | 89 | 83 | 63 | 66 |
| 5 | 100 (197 562) | 108 | 107 | 117 | 85 | 84 | 76 |
| 6 | 100 (178 934) | 116 | 116 | 105 | 85 | 73 | 56 |
| 7 | 100 (172 653) | 111 | 108 | 112 | 83 | 76 | 53 |
| Patient no. . | % untreated control (dpm/107 cells) . | RNA synthesis, % of control . | |||||
|---|---|---|---|---|---|---|---|
| rGel . | rGel/BlyS . | ||||||
| 1.0 μM . | 2.5 μM . | 5.0 μM . | 1.0 nM . | 2.5 nM . | 5.0 nM . | ||
| 1 | 100 (72 419) | 126 | 106 | 110 | ND | ND | ND |
| 2 | 100 (173 833) | 99 | 99 | 101 | 130 | 29 | 72 |
| 3 | 100 (116 060) | 125 | 99 | 59 | 69 | 79 | 57 |
| 4 | 100 (188 671) | 104 | 88 | 89 | 83 | 63 | 66 |
| 5 | 100 (197 562) | 108 | 107 | 117 | 85 | 84 | 76 |
| 6 | 100 (178 934) | 116 | 116 | 105 | 85 | 73 | 56 |
| 7 | 100 (172 653) | 111 | 108 | 112 | 83 | 76 | 53 |
Primary CLL cells from the patients 1 to 7 were isolated and incubated with either 1.0, 2.5, and 5.0 μM rGel or 1.0, 2.5, and 5.0 nM rGel/BLyS for 72 hours and pulsed with [3H]uridine for 1 hour. At the end of incubation the uridine incorporation into RNA was measured. The values are expressed as percentage of control (untreated) cells.