Modules of retrovival vectors used in this study
| Vector . | LTR . | cDNA . | 3′UTR . |
|---|---|---|---|
| SF91DsRed2 | SFFVp | DsRed2 | wPRE |
| SF91EGFP | SFFVp | EGFP | wPRE |
| SF91IRESEGFP | SFFVp | IRES-EGFP | wPRE |
| SF91XRCC4 | SFFVp | XRCC4-IRES-EGFP | wPRE |
| SF11flCD34 | SFFVp | flCD34 | — |
| SF11tCD34 | SFFVp | tCD34 | — |
| SF11mflCD34 | SFFVp | mflCD34 | — |
| SF11mtCD34 | SFFVp | mtCD34 | — |
| SF11dLNGFR | SFFVp | dLNGFR | — |
| HaMDR | HaMSV | MDR1 | VL30 |
| SF91TAg | SFFVp | EGFP2ASV40TAg | — |
| Vector . | LTR . | cDNA . | 3′UTR . |
|---|---|---|---|
| SF91DsRed2 | SFFVp | DsRed2 | wPRE |
| SF91EGFP | SFFVp | EGFP | wPRE |
| SF91IRESEGFP | SFFVp | IRES-EGFP | wPRE |
| SF91XRCC4 | SFFVp | XRCC4-IRES-EGFP | wPRE |
| SF11flCD34 | SFFVp | flCD34 | — |
| SF11tCD34 | SFFVp | tCD34 | — |
| SF11mflCD34 | SFFVp | mflCD34 | — |
| SF11mtCD34 | SFFVp | mtCD34 | — |
| SF11dLNGFR | SFFVp | dLNGFR | — |
| HaMDR | HaMSV | MDR1 | VL30 |
| SF91TAg | SFFVp | EGFP2ASV40TAg | — |
A high MOI was used in some experiments involving vectors HaMDR1 and SF91dsRed2.5
SFFV indicates spleen focus-forming virus; HaMLV, Harvey murine leukemia virus; EGFP, enhanced green fluorescent protein; DsRed2, red fluorescent protein; IRES-EGFP, internal ribosomal entry site followed by EGFP; XRCC4, x-ray repair complementing defective repair in Chinese hamster cells 4; flCD34, human full-length CD34; —, no additional element in 3′ UTR; tCD34, human truncated CD34; mflCD34, murine full-length CD34; mtCD34, murine truncated CD34; dLNGFR, deleted low-affinity nerve growth factor receptor; MDR1, multidrug resistance 1; EGFP2ASV40TAg, EGFP fusion protein linked to the large T antigen (TAg) of simian virus 40 using a self-cleaving 2A proteinase sequence; VL30, virus-like element 30; wPRE, woodchuck hepatitis virus posttranscriptional regulatory element.